Phenotype Help

NET1 / YJL076W Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.



Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

25 entries for 15 phenotypes


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

PhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetailsReference
autophagy: increased
classical geneticsconditional
Allele: net1-1
W303Temperature: elevated temperature, 37 °C
Details: autophagy monitored by processing of GFP-Atg8p
Kondo A, et al. (2018) PMID:29694832
chemical compound excretion: increased
systematic mutation set

Dox titratable strains: Tet-regulated promoter that is shut off by the addition of doxycycline

repressibleS288C inositolDetails: Opi- phenotype; overproduction and excretion of inositol in the absence of inositol and choline
Salas-Santiago B and Lopes JM (2014) PMID:24558266
chromosome segregation: decreased
homozygous diploid reduction of function
Allele: net1-6Cdk

T62A, S166A, T212A, S252A, T297A, T304A; all Cdk-consensus sites within amino acids 1-341 mutated

W303Phase: meiosis I
Details: inhibition of rDNA segregation during meiosis I; fail to disjoin their rDNA while traversing meiosis I and remains in a single mass into meiotic metaphase II
Yellman CM (2023) PMID:37530060
chromosome segregation: delayed
classical genetics reduction of function
Allele: net1-6Cdk

T62A, S166A, T212A, S252A, T297A, T304A; all Cdk consensus sites within amino acids 1-341 mutated

W303Details: 10 minute delay in the segregation of ribosomal DNA (rDNA) during mitosis
Azzam R, et al. (2004) PMID:15273393
competitive fitness: decreased
competitive growth

fitness profiling of essential genes using hypomorphic DAmP alleles

reduction of functionS288CMedia: minimal medium
Details: Relative fitness score: 0.998
Breslow DK, et al. (2008) PMID:18622397
growth in exponential phase: decreased rate
classical genetics unspecified
Allele: net1-T62A,S385A

T62A, S385A; Hog1p phosphorylation sites mutated

W3031 M sodium chlorideTreatment: metaphase-block release
Details: osmosensitive; decreased recovery and osmoadaptation in metaphase-synchronized cells relative to wt
Tognetti S, et al. (2020) PMID:32265285
haploinsufficient
heterozygous diploid, competitive growth

genome-wide fitness profiling

null
Allele: net1-Δ
S288CMedia: turbidostat growth in FPM medium
Details: Relative growth score: -0.0063
Pir P, et al. (2012) PMID:22244311
haploinsufficient
heterozygous diploid, systematic mutation set null
Allele: net1-Δ
S288COhnuki S and Ohya Y (2018) PMID:29768403
inviable
systematic mutation setnull
Allele: net1-Δ
S288CGiaever G, et al. (2002) PMID:12140549
protein/peptide accumulation: increased
Reporter: Mob1
classical genetics activationW303Details: increased localization to nucleus prior to anaphase
Zhou X, et al. (2021) PMID:33481703
Showing 1 to 10 of 25 entries

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).


Reset

Click on a gene or phenotype observable name to go to its specific page within SGD; drag any of the gene or observable objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram; filter the genes that share observable terms with the given gene by the number of terms they share by clicking anywhere on the slider bar or dragging the tab to the desired filter number.


Resources