Phenotype Help

DUO1 / YGL061C Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.


Summary
Essential gene; conditional mutations and overexpression result in chromosome instability

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

21 entries for 13 phenotypes


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

PhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetailsReference
chromosome/plasmid maintenance: decreased
systematic mutation setoverexpressionOtherAssay: a-like faker (ALF) assay
Details: dosage chromosome instability (dCIN) involving chromosome loss, rearrangement and/or gene conversion events
Duffy S, et al. (2016) PMID:27551064
chromosome/plasmid maintenance: decreased
systematic mutation setoverexpressionOtherAssay: chromosome transmission fidelity (CTF) assay
Details: dosage chromosome instability (dCIN) involving chromosome loss
Duffy S, et al. (2016) PMID:27551064
cold sensitivity: increased
classical geneticsreduction of function
Allele: duo1-S115F
OtherTemperature: reduced temperature, 14 °C
Robinson LC, et al. (2012) PMID:23275890
colony sectoring: increased
large-scale surveyconditional
Allele: duo1-2
S288CAssay: chromosome transmission fidelity (CTF) assay
Temperature: semi-permissive temperature
Details: indicative of chromosome instability (CIN); metascore weak (single hit)
Stirling PC, et al. (2011) PMID:21552543
competitive fitness: decreased
competitive growth

fitness profiling of essential genes using hypomorphic DAmP alleles

reduction of functionS288CMedia: minimal medium
Details: Relative fitness score: 0.991
Breslow DK, et al. (2008) PMID:18622397
competitive fitness: decreased
competitive growth overexpressionS288CDetails: decreased abundance within the barFLEX overexpression collection arrayed after 20 generations of pooled growth
Douglas AC, et al. (2012) PMID:23050238
haploinsufficient
heterozygous diploid, competitive growth

genome-wide fitness profiling

null
Allele: duo1-Δ
S288CMedia: turbidostat growth in FPM medium
Details: Relative growth score: -0.0034
Pir P, et al. (2012) PMID:22244311
invasive growth: increased
homozygous diploid, systematic mutation setoverexpressionSigma1278bMedia: nitrogen-sufficient medium
Details: agar invasion score: 0.97; enhanced pseudohyphal growth, based on elevated invasiveness
Shively CA, et al. (2013) PMID:23410832
inviable
systematic mutation setnull
Allele: duo1-Δ
S288CGiaever G, et al. (2002) PMID:12140549
nucleolar morphology: abnormal
systematic mutation set conditional
Allele: duo1-2

A117T, M124I

S288CTemperature: elevated temperature, 37 °C
Details: increased nucleolar fragmentation; high confidence candidate
Neumüller RA, et al. (2013) PMID:23962978
Showing 1 to 10 of 21 entries

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).


Reset

Click on a gene or phenotype observable name to go to its specific page within SGD; drag any of the gene or observable objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram; filter the genes that share observable terms with the given gene by the number of terms they share by clicking anywhere on the slider bar or dragging the tab to the desired filter number.

1011

Resources


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GLC7