Phenotype Help

BIL2 / YGL015C Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.


Summary
Non-essential gene; null mutant shows abnormal actin cytoskeleton morphology and vesicle distribution; in systematic studies, overexpression decreases UV resistance

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

19 entries for 13 phenotypes


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PhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetailsReference
actin cytoskeleton morphology: abnormal
classical genetics null
Allele: bil2-Δ
W303Details: visible disorganization of actin cable networks with an increased number of cable segments in mother cells, but minimal effects on the polarized distribution cortical actin patches
Rands TJ and Goode BL (2021) PMID:33634134
actin cytoskeleton morphology: abnormal
classical genetics null
Allele: bil2-Δ
W303Treatment: α-factor, 100 μM
Details: increase in cable tortuosity (length/distance) and actin cable entangled during pheromone-induced mating projection formation
Magliozzi JO, et al. (2024) PMID:38656798
cell cycle progression in G1 phase: increased duration
homozygous diploid, systematic mutation setnull
Allele: bil2-Δ
S288CDetails: percentage of G1 cells greater than two standard deviations (41.57%) above wild type when assayed by FACS
Hoose SA, et al. (2012) PMID:22438835
competitive fitness: increased
competitive growth

fitness profiling using complete deletion alleles

null
Allele: bil2-Δ
S288CMedia: minimal medium
Details: Relative fitness score: 1.008
Breslow DK, et al. (2008) PMID:18622397
mating projection morphology: abnormal
classical genetics null
Allele: bil2-Δ
W303Treatment: α-factor, 100 μM
Details: prematurely form second and a third mating projections after exposure to high concentrations of α-factor for 3 hours; projections are slightly shorter than wt; defective in committing to a single polarity axis at a time; exhibit repetitive shmooing behavior
Magliozzi JO, et al. (2024) PMID:38656798
nuclear position: abnormal
classical genetics null
Allele: bil2-Δ
W303Treatment: α-factor, 100 μM
Details: nuclear position is compromised in the null mutant during mating projection formation, implicating actin cable architecture for nuclear migration into the tip of the mating projection
Magliozzi JO, et al. (2024) PMID:38656798
protein/peptide distribution: abnormal
Reporter: Bni1-GFP
classical genetics null
Allele: bil2-Δ
W303Treatment: α-factor, 100 μM
Details: Bni1p prematurely disappears from the first mating projection tip; remnants remain at the first mating projection, with a less tightly focused signal on the cell cortex where the new mating projection will emerge
Magliozzi JO, et al. (2024) PMID:38656798
protein/peptide distribution: abnormal
Reporter: Fus-Mid-GFP

extracellular O-glycosylated region of Fus1p fused to the transmembrane domain and cytoplasmic tail of Mid2p

classical geneticsnull
Allele: bil2-Δ
S288CDetails: accumulates in the Golgi in addition to the plasma membrane
Proszynski TJ, et al. (2005) PMID:16330752
protein/peptide distribution: abnormal
Reporter: Fus-Mid-GFP

extracellular O-glycosylated region of Fus1p fused to the transmembrane domain and cytoplasmic tail of Mid2p

systematic mutation setnull
Allele: bil2-Δ
S288CDetails: reporter displays dot-like intracellular localization; vacuolar staining is absent; delivery to the plasma membrane is normal or enhanced
Proszynski TJ, et al. (2005) PMID:16330752
resistance to chemicals: decreased
homozygous diploid, systematic mutation setnull
Allele: bil2-Δ
S288C benzo[a]pyreneO'Connor ST, et al. (2012) PMID:23403841
Showing 1 to 10 of 19 entries

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).


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