SPS1 / YDR523C Overview

Standard Name
SPS1 1
Systematic Name
Feature Type
ORF , Verified
STE20-family GCKIII protein kinase; required for prospore membrane closure, efficient spore packaging, and localization of enzymes involved in spore wall synthesis; component of a non-canonical Hippo pathway with Cdc15p involved in spindle disassembly and sustained release of Cdc14p during anaphase II; forms a complex with Cdc15p; phosphorylation requires Cdc15p; required for Ssp1p phosphorylation and turnover; interacts with 14-3-3 proteins; expressed in late meiosis; nuclear and cytoplasmic 2 3 4 5 6
Name Description
SPorulation Specific 1
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

SPS1 is located on the right arm of chromosome IV between SPS2 sporulation protein and AGE1 ARF GAP; replication origin ARS446 overlaps 5' end of SPS1; coding sequence is 1473 nucleotides long with 7 SNPs, 2 of which cause amino acid polymorphisms
Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Sps1p is 490 amino acids long, low in abundance; phosphorylated on 3 serines
Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.

View all SPS1 alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Putative protein kinase involved in positive regulation of prospore membrane formation; has been shown to negatively regulate localization of Cdc14p to the nucleolus during meiotic anaphase II; localized to prospore membrane, nucleus and cytoplasm

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated
Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Non-essential gene; reduced function alleles compromise sporulation; null grows slowly, cannot respire, is sensitive to various chemicals, has increased lifespan, hyperelongated prospore membranes during late anaphase II, delayed appearance and accumulation of closed prospore membranes, abnormal spindles, increased resistance to oxidative stress
Disease Details


Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.

Yeast SPS1 is homologous to human STRADA, and has been used to study mutations found in patients with a rare neurodevelopmental disorder characterized by polyhydramnios, megalencephaly, and symptomatic epilepsy (PMSE syndrome)

Manually Curated

Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

Sps1p interacts physically with proteins involved in meiotic cell cycle and sporulation; SPS1 interacts genetically with genes involved in transcription

123 total interactions for 108 unique genes

Physical Interactions

  • Affinity Capture-MS: 26
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 5
  • Biochemical Activity: 6
  • Co-localization: 1
  • Reconstituted Complex: 22
  • Two-hybrid: 3

Genetic Interactions

  • Dosage Growth Defect: 8
  • Dosage Lethality: 1
  • Negative Genetic: 33
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 4
  • Positive Genetic: 3
  • Synthetic Growth Defect: 4
  • Synthetic Rescue: 1
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

SPS1 transcription is regulated by Sfp1p in response to stress; SPS1 transcription is upregulated by Ndt80p during sporulation; Sps1 protein activity is regulated by Cdc15p during sporulation
Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.

Last Updated: 2024-06-14

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.