Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.
A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.
23 entries for 11 phenotypesIncrease the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.
| Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details | Reference |
|---|---|---|---|---|---|---|
| competitive fitness: decreased | competitive growth fitness profiling using complete deletion alleles | null Allele: rqc1-Δ | S288C | Media: minimal medium Details: Relative fitness score: 0.99 | Breslow DK, et al. (2008) PMID:18622397 | |
| competitive fitness: decreased | systematic mutation set | null Allele: rqc1-Δ | S288C | Media: rich medium (YPD) w/6% ethanol, ETH | Qian W, et al. (2012) PMID:23103169 | |
| competitive fitness: decreased | systematic mutation set | null Allele: rqc1-Δ | S288C | Media: synthetic complete medium, SC | Qian W, et al. (2012) PMID:23103169 | |
| haploproficient | heterozygous diploid, competitive growth genome-wide fitness profiling | null Allele: rqc1-Δ | S288C | Media: turbidostat growth in FPM medium Details: Relative growth score: 0.0033 | Pir P, et al. (2012) PMID:22244311 | |
| heat sensitivity: increased | homozygous diploid, systematic mutation set pooled screening for fitness defects at 37 deg C relative to 30 deg C | null Allele: rqc1-Δ | S288C | Temperature: elevated temperature, 37 °C | Sinha H, et al. (2008) PMID:18780730 | |
| invasive growth: increased | homozygous diploid, systematic mutation set | overexpression | Sigma1278b | Media: nitrogen-sufficient medium Details: agar invasion score: 0.97; enhanced pseudohyphal growth, based on elevated invasiveness | Shively CA, et al. (2013) PMID:23410832 | |
| protein/peptide accumulation: increased Reporter: PtnA NS-Rz | classical genetics | null Allele: rqc1-Δ | S288C | Details: increased stalled nacent chain aggregates observed | Yonashiro R, et al. (2016) PMID:26943317 | |
| protein/peptide accumulation: increased Reporter: TAP-Non-Stop protein increased accumulation of aberrant non-stop proteins on 80S ribosomes | classical genetics | null Allele: rqc1-Δ | S288C | Details: increased accumulation of aberrant non-stop proteins on 60S ribosomes also observed | Defenouillère Q, et al. (2013) PMID:23479637 | |
| replicative lifespan: increased | systematic mutation set | null Allele: rqc1-Δ | S288C | Details: long-lived mutants, one standard deviation above the mean, were identified using an HTP sequencing-based yeast replicative lifespan screen (SEBYL), with pooled cells subject to four rounds of biotin-based sorting to enrich old vs young cells, followed by sequencing to ID mutants and abundance | Yu R, et al. (2021) PMID:33790287 | |
| resistance to chemicals: decreased | systematic mutation set | null Allele: rqc1-Δ | S288C | 10 mg/ml paromomycin | Alamgir M, et al. (2010) PMID:20691087 |
This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).
Click on a gene or phenotype observable name to go to its specific page within SGD; drag any of the gene or observable objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram; filter the genes that share observable terms with the given gene by the number of terms they share by clicking anywhere on the slider bar or dragging the tab to the desired filter number.
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