NGG1 / YDR176W Overview

Standard Name
NGG1 1
Systematic Name
ADA3 19 , SWI7 20
Feature Type
ORF , Verified
Subunit of chromatin modifying histone acetyltransferase complexes; member of the ADA complex, the SAGA complex, and the SLIK complex; transcriptional regulator involved in glucose repression of Gal4p-regulated genes 1 2 3 4
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)
2457 +/- 349
Half-life (hr)


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.

View all NGG1 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Subunit of the SAGA, SLIK, and Ada2/Gcn5/Ada3 histone acetyltransferase complexes involved in chromatin modification and transcription activation

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated


Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.

Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Non-essential gene; null mutants grow slowly, have shortened lifespan, decreased fitness and ethanol tolerance, abnormal morphology of vacuoles and buds, are sensitive to preservatives, antibiotics, heat, osmotic stress, dessication; homozygous diploid nulls cannot sporulate; overexpression affects chromatin silencing
Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

462 total interactions for 178 unique genes

Physical Interactions

  • Affinity Capture-MS: 134
  • Affinity Capture-RNA: 5
  • Affinity Capture-Western: 81
  • Biochemical Activity: 1
  • Co-fractionation: 6
  • Co-purification: 45
  • PCA: 1
  • Reconstituted Complex: 11
  • Two-hybrid: 14

Genetic Interactions

  • Dosage Lethality: 1
  • Negative Genetic: 68
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 6
  • Synthetic Growth Defect: 66
  • Synthetic Lethality: 19
  • Synthetic Rescue: 2
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

NGG1 (also called ADA3) encodes a component of three chromatin modifying histone acetyltransferase (HAT) complexes: SAGA, SLIK, and ADA. These complexes function in positive and negative transcriptional regulation of numerous RNA polymerase II-transcribed genes; in addition, SLIK plays a role in the retrograde response. The three complexes each contain the histone acetyltransferase catalytic subunit Gcn5p, which interacts directly with Ada2p and preferentially modifies histones H3 and H2B. In vitro, Gcn5p acetylates N-terminal lysines on free histones, but acetylation of nucleosomal histone substrates also requires Ada2p and Ngg1p, which are found in a complex with Gcn5p. Ada2p has been shown to increase the HAT activity of Gcn5p, while Ngg1p plays a role in expanding the range of lysines that undergo acetylation.
Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.

Last Updated: 2010-03-26

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.