Phenotype Help

AIM21 / YIR003W Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.


Summary
Non-essential gene; null mutant displays an increased frequency of spontaneous mitochondrial genome loss; null mutant is slightly heat sensitive; null mutant displays increased survival following a ramped heat stimulus, but decreased resistance to starvation stress; null mutant displays increased biofilm formation, and has an endocytosis defect; heterozygous diploid null is haploinsufficient

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

27 entries for 16 phenotypes


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

PhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetailsReference
actin cytoskeleton morphology: abnormal
classical geneticsnull
Allele: aim21-Δ
S288CDetails: reduced numbers of actin cables
Shin M, et al. (2018) PMID:29467252
biofilm formation: increased
systematic mutation setnull
Allele: aim21-Δ
S288CVandenbosch D, et al. (2013) PMID:24034557
competitive fitness: decreased
systematic mutation setnull
Allele: aim21-Δ
S288CMedia: synthetic complete medium, SC
Qian W, et al. (2012) PMID:23103169
competitive fitness: increased
competitive growth

fitness profiling using complete deletion alleles

null
Allele: aim21-Δ
S288CMedia: minimal medium
Details: Relative fitness score: 1.001
Breslow DK, et al. (2008) PMID:18622397
endocytosis: decreased
systematic mutation setnull
Allele: aim21-Δ
S288CDetails: defective internalization of a chimeric Snc1p reporter, GFP-Snc1-Suc2
Burston HE, et al. (2009) PMID:19506040
endocytosis: decreased
Reporter: lucifer yellow
classical geneticsnull
Allele: aim21-Δ
S288CDetails: small but significant defect in fluid-phase endocytosis
Farrell KB, et al. (2017) PMID:28706108
endocytosis: delayed
Reporter: Mup1-GFP
classical geneticsnull
Allele: aim21-Δ
S288CMedia: methionine-rich media
Details: delayed internalization of the Mup1p transporter in the presence of methionine
Farrell KB, et al. (2017) PMID:28706108
haploinsufficient
heterozygous diploid, competitive growth

genome-wide fitness profiling

null
Allele: aim21-Δ
S288CMedia: turbidostat growth in FPM medium
Details: Relative growth score: -0.0035
Pir P, et al. (2012) PMID:22244311
heat sensitivity: decreased
systematic mutation set

high throughput heat ramp cell death assay

null
Allele: aim21-Δ
S288CTemperature: elevated temperature
Details: ramp temperature from ambient to 30 deg C immediately, hold 1 min at 30 deg C, ramp temperature from 30 deg C to 62 deg C over 20 min, return to ambient
Teng X, et al. (2011) PMID:21814286
heat sensitivity: increased
systematic mutation setnull
Allele: aim21-Δ
S288CTemperature: elevated temperature, 39 °C
Details: weak heat-sensitivity; formation of smaller colonies than WT
Ruiz-Roig C, et al. (2010) PMID:20398213
Showing 1 to 10 of 27 entries

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).


Reset

Click on a gene or phenotype observable name to go to its specific page within SGD; drag any of the gene or observable objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram; filter the genes that share observable terms with the given gene by the number of terms they share by clicking anywhere on the slider bar or dragging the tab to the desired filter number.


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