CST6 / YIL036W Overview
- Standard Name
- CST6 1
- Systematic Name
- YIL036W
- SGD ID
- SGD:S000001298
- Aliases
- SHF1 8 , ACA2 3
- Feature Type
- ORF , Verified
- Description
- Basic leucine zipper (bZIP) transcription factor from ATF/CREB family involved in stress-responsive regulatory network; mediates transcriptional activation of NCE103 in response to low CO2 levels; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; relocalizes to the cytosol in response to hypoxia; CST6 has a paralog, ACA1, that arose from the whole genome duplication 1 2 3 4 5 6 7
- Name Description
- Chromosome STability 1
- Paralog
- ACA1 6
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- CST6 has a paralog, ACA1, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Relocalizes to the cytosol in response to hypoxia
- Length (a.a.)
- 587
- Mol. Weight (Da)
- 65266.2
- Isoelectric Point
- 8.22
- Median Abundance (molecules/cell)
- 2514 +/- 1274
- Half-life (hr)
- 5.9
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.
View all CST6 alleles in SGD search | YeastMine
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Sequence-specific DNA binding RNA polymerase II transcription factor involved in the cellular responses to carbon dioxide and oleic acid
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in cellular response to carbon dioxide (IMP)
- involved in cellular response to oleic acid (IMP)
- involved in cellular response to stress (IDA)
- involved in positive regulation of transcription by RNA polymerase II (IDA)
- involved in positive regulation of transcription from RNA polymerase II promoter by oleic acid (IMP)
- involved in regulation of transcription by RNA polymerase II (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant is slow-growing and cold-sensitive, also displays increased sensitivity to oxidative stress and ethanol, but increased resistance to benomyl; overexpression results in elevated mitotic recombination; in systematic studies mutants exhibit reduced competitive fitness
Classical Genetics
- overexpression
- null
- unspecified
- null
- auxotrophy
- chemical compound accumulation: increased
- chronological lifespan: decreased
- competitive fitness: decreased
- heat sensitivity: increased
- oxidative stress resistance: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: absent
- respiratory growth: decreased rate
- stress resistance: decreased
- telomere length: decreased
- toxin resistance: increased
- transposable element transposition: decreased
- vacuolar morphology: abnormal
- vegetative growth: decreased
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The cst6 null mutant is viable; the null mutant of paralog aca1 is viable; the cst6 aca1 double mutant is viable.
215 total interactions for 202 unique genes
Physical Interactions
- Affinity Capture-MS: 38
- Affinity Capture-RNA: 6
- Biochemical Activity: 2
- Co-purification: 1
- PCA: 1
- Proximity Label-MS: 1
- Reconstituted Complex: 1
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Rescue: 3
- Negative Genetic: 124
- Phenotypic Suppression: 1
- Positive Genetic: 30
- Synthetic Growth Defect: 5
- Synthetic Rescue: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- CST6 encodes a transcriptional activator of the ATF/CREB family, containing a bZIP structural motif which consists of a leucine zipper that mediates dimerization and an adjacent basic region that contacts DNA. Cst6p binds as a homodimer to the ATF/CREB consensus sequence TGACGTCA, and with comparable efficiency to ATF/CREB sites with single or double substitutions at positions +/- 2. Cst6p can also bind ATF/CREB sites as a heterodimer with Aca1p. Cst6p is important for a variety of biological processes including growth on nonoptimal carbon sources and resistance to a variety of drugs. Target genes include GRE2 and COS8. Cst6p-dependent activation is regulated to some extent by carbon source.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.