RIM101 / YHL027W Overview

Standard Name
RIM101 1
Systematic Name
Feature Type
ORF , Verified
Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression; involved in cell wall assembly; plays role in lager yeast flocculation under brewing conditions; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC 2 3 4 5 6
Name Description
Regulator of IME2 1
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)
1603 +/- 506
Half-life (min)


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.

View all RIM101 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Sequence-specific DNA binding transcription factor that regulates transcription by RNA polymerase II involved in meiosis, spore formation, cell wall biosynthesis, and the cellular responses to anoxia and alkaline pH

View computational annotations

Cellular Component

Manually Curated
Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Non-essential gene; null mutants have abnormal vacuolar morphology, are impaired in respiration, endocytosis, sporulation, filamentous growth, and biofilm formation; nulls are also sensitive to various toxins, antibiotics, and acid pH, and are resistant to metals; conditional mutants are sensitive to cold; overexpression increases resistance to TOR inhibitor rapamycin
Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

650 total interactions for 482 unique genes

Physical Interactions

  • Affinity Capture-MS: 6
  • Affinity Capture-RNA: 5
  • Affinity Capture-Western: 1
  • Biochemical Activity: 2
  • Co-localization: 1
  • PCA: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 4

Genetic Interactions

  • Dosage Rescue: 17
  • Negative Genetic: 430
  • Phenotypic Enhancement: 7
  • Phenotypic Suppression: 30
  • Positive Genetic: 58
  • Synthetic Growth Defect: 59
  • Synthetic Lethality: 15
  • Synthetic Rescue: 13
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

RIM101 encodes a transcriptional repressor that is a member of the C6 zinc finger class, containing a DNA binding domain also known as the Zn2Cys6 binuclear zinc cluster or zinc knuckle. Two of the major targets of Rim101p repression are NRG1 and NRG2, encoding transcriptional repressors. Rim101p regulates a variety of processes, either directly or indirectly via repression of NRG1 and NRG2: response to alkaline pH, weak acid stress, and anaerobic conditions; filamentous growth; meiosis and sporulation; septum assembly; response to altered lipid asymmetry in the plasma membrane bilayer; and cell wall assembly. Rim101p represses transcription by recruiting the Cyc8p-Tup1p complex to promoters. Rim101p activity is regulated by proteolysis. In acidic conditions, Rim101p exists as a full-length, inactive form. At high pH, the C-terminal tail is removed by Rim13p protease, activating Rim101p as a transcriptional repressor. Nuclear localization of Rim101p is also regulated, via phosphorylation. This regulation is dependent on Pho85p kinase but it is not known whether Rim101p is a direct target of Pho85p in vivo.
Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.