RIM101 / YHL027W Overview
- Standard Name
- RIM101 1
- Systematic Name
- YHL027W
- SGD ID
- SGD:S000001019
- Aliases
- RIM1
- Feature Type
- ORF , Verified
- Description
- Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression; involved in cell wall assembly; plays role in lager yeast flocculation under brewing conditions; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC 2 3 4 5 6
- Name Description
- Regulator of IME2 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 625
- Mol. Weight (Da)
- 68231.2
- Isoelectric Point
- 7.23
- Median Abundance (molecules/cell)
- 1603 +/- 506
- Half-life (min)
- 42.8
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all RIM101 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Sequence-specific DNA binding transcription factor that regulates transcription by RNA polymerase II involved in meiosis, spore formation, cell wall biosynthesis, and the cellular responses to anoxia and alkaline pH
View computational annotations
Molecular Function
- Manually Curated
- enables DNA-binding transcription repressor activity, RNA polymerase II-specific (IMP, IGI, IDA)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in ascospore formation (IMP)
- involved in cellular response to alkaline pH (IMP)
- involved in cellular response to anoxia (IMP)
- involved in fungal-type cell wall biogenesis (IGI, IMP)
- involved in meiotic cell cycle (IMP)
- involved in negative regulation of transcription by RNA polymerase II (IDA)
- involved in positive regulation of division septum assembly (IGI)
- involved in positive regulation of transcription by RNA polymerase II (IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutants have abnormal vacuolar morphology, are impaired in respiration, endocytosis, sporulation, filamentous growth, and biofilm formation; nulls are also sensitive to various toxins, antibiotics, and acid pH, and are resistant to metals; conditional mutants are sensitive to cold; overexpression increases resistance to TOR inhibitor rapamycin
Classical Genetics
- null
- acid pH resistance: decreased
- autophagy: decreased
- biofilm formation: decreased
- chemical compound accumulation: decreased
- invasive growth: absent
- invasive growth: decreased
- metal resistance: decreased
- metal resistance: increased
- protein/peptide accumulation: decreased
- protein/peptide accumulation: increased
- protein/peptide modification: decreased
- pseudohyphal growth: absent
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: decreased
- RNA accumulation: decreased
- RNA accumulation: increased
- utilization of carbon source: decreased
- viability: decreased
- conditional
- unspecified
- null
- acid pH resistance: decreased
- biofilm formation: decreased
- chemical compound accumulation: increased
- chronological lifespan: increased
- colony appearance: abnormal
- competitive fitness: decreased
- competitive fitness: increased
- endocytosis: decreased
- haploinsufficient
- invasive growth: absent
- liquid culture appearance: abnormal
- metal resistance: decreased
- nutrient utilization: increased
- oxidative stress resistance: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: absent
- RNA accumulation: decreased
- sporulation efficiency: decreased
- sporulation: decreased
- stress resistance: increased
- toxin resistance: decreased
- utilization of nitrogen source: decreased rate
- vacuolar morphology: abnormal
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
651 total interactions for 483 unique genes
Physical Interactions
- Affinity Capture-MS: 6
- Affinity Capture-RNA: 6
- Affinity Capture-Western: 1
- Biochemical Activity: 2
- Co-localization: 1
- PCA: 1
- Reconstituted Complex: 1
- Two-hybrid: 4
Genetic Interactions
- Dosage Rescue: 17
- Negative Genetic: 430
- Phenotypic Enhancement: 7
- Phenotypic Suppression: 30
- Positive Genetic: 58
- Synthetic Growth Defect: 59
- Synthetic Lethality: 15
- Synthetic Rescue: 13
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- RIM101 encodes a transcriptional repressor that is a member of the C6 zinc finger class, containing a DNA binding domain also known as the Zn2Cys6 binuclear zinc cluster or zinc knuckle. Two of the major targets of Rim101p repression are NRG1 and NRG2, encoding transcriptional repressors. Rim101p regulates a variety of processes, either directly or indirectly via repression of NRG1 and NRG2: response to alkaline pH, weak acid stress, and anaerobic conditions; filamentous growth; meiosis and sporulation; septum assembly; response to altered lipid asymmetry in the plasma membrane bilayer; and cell wall assembly. Rim101p represses transcription by recruiting the Cyc8p-Tup1p complex to promoters. Rim101p activity is regulated by proteolysis. In acidic conditions, Rim101p exists as a full-length, inactive form. At high pH, the C-terminal tail is removed by Rim13p protease, activating Rim101p as a transcriptional repressor. Nuclear localization of Rim101p is also regulated, via phosphorylation. This regulation is dependent on Pho85p kinase but it is not known whether Rim101p is a direct target of Pho85p in vivo.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.