BMH1 / YER177W Overview


Standard Name
BMH1 1
Systematic Name
YER177W
SGD ID
SGD:S000000979
Aliases
APR6
Feature Type
ORF , Verified
Description
14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of exocytosis, vesicle transport, Ras/MAPK and rapamycin-sensitive signaling, aggresome formation, spindle position checkpoint; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; antiapoptotic gene similar to human 14-3-3; BMH1 has a paralog, BMH2, that arose from whole genome duplication 2 3 4 5 6 7 8
Name Description
Brain Modulosignalin Homolog 1
Paralog
BMH2 5
Comparative Info
Integrated model organism details available at the Alliance of Genome Resources website
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
267
Mol. Weight (Da)
30074.6
Isoelectric Point
4.54
Median Abundance (molecules/cell)
65471 +/- 30341
Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Phosphoserine- and DNA replication origin-binding protein involved in many signaling pathways and processes including the DNA damage and spindle position checkpoints, signal transduction during filamentous growth, cell wall chitin biosynthesis, and aggresome assembly; localizes to the nucleus, cytoplasm, and cytoplasmic stress granule

View computational annotations

Cellular Component

Manually Curated
High-Throughput
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null mutation results in increased frequency of gross chromosomal rearrangements, lengthened chronological lifespan, decreased sensitivity to desiccation and increased innate thermotolerance; null mutant is sensitive to hyperosmotic stress and resistant to oxidative stress; overexpression leads to slow growth
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
The bmh1 null mutant is viable; the null mutant of paralog bmh2 is viable; the bmh1 bmh2 double mutant is inviable or displays a growth defect.

752 total interactions for 477 unique genes

Physical Interactions

  • Affinity Capture-MS: 139
  • Affinity Capture-RNA: 7
  • Affinity Capture-Western: 35
  • Biochemical Activity: 1
  • Co-crystal Structure: 1
  • Co-fractionation: 1
  • Co-purification: 3
  • Far Western: 1
  • PCA: 6
  • Protein-peptide: 4
  • Reconstituted Complex: 15
  • Two-hybrid: 12

Genetic Interactions

  • Dosage Growth Defect: 14
  • Dosage Lethality: 1
  • Dosage Rescue: 15
  • Negative Genetic: 321
  • Phenotypic Enhancement: 20
  • Phenotypic Suppression: 8
  • Positive Genetic: 75
  • Synthetic Growth Defect: 38
  • Synthetic Lethality: 17
  • Synthetic Rescue: 18
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Regulators
16
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.


Last Updated: 1999-09-01

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
88
Additional
66
Reviews
43

Resources