BEM2 / YER155C Overview

Standard Name
BEM2 1 2
Systematic Name
IPL2 8 , SUP9 , TSL1
Feature Type
ORF , Verified
Rho GTPase activating protein (RhoGAP); contains RhoGAP activity specific for both Rho1p and Cdc42p, and potentially Rho4p; acts with the RhoGAP Bem3p to negatively regulate Cdc42p-dependent signal transduction; involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence and for the morphogenesis checkpoint in response to actin depolymerization; localizes to sites of polarized growth and the plasma membrane 1 3 4 5 6 7
Name Description
Bud EMergence 2
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)
4005 +/- 1315
Half-life (hr)


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.

View all BEM2 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

GTPase activator involved in actin cytoskeleton organization and negative regulation of Rho protein signal transduction; localizes to many places throughout cell, including prospore membrane, incipient bud site, bud tip, shmoo tip, plasma membrane, cytoplasm and mitochondria

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated
Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Non-essential gene; null mutant has a competitive fitness defect, a decreased growth rate in rich media and a severe respiratory growth defect; null and conditional mutants are heat sensitive, arresting as large, multinucleate cells, with some polyploid and aploid cells; null mutant is defective in polarization of the actin cytoskeleton, with reduced endocytosis; null mutant accumulates hyperphosphorylated Cdc24p at multiple cortical sites, displays increased Cdc42p activation, a reduction in Gic2p levels and elevated Slt2p phosphoryation in the absence of stress; null mutant displays increased chitin deposition, and mitophagy; null mutant is zymolyase sensitive; null mutant is sensitive to benomyl, caffeine, cell wall agents and zymolyase as well as to some DNA replication inhibitors and damaging agents; heterozygous null mutant is haploproficient
Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

955 total interactions for 598 unique genes

Physical Interactions

  • Affinity Capture-MS: 88
  • Affinity Capture-RNA: 8
  • Affinity Capture-Western: 1
  • Biochemical Activity: 2
  • Co-fractionation: 2
  • Co-localization: 1
  • Co-purification: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 5

Genetic Interactions

  • Dosage Rescue: 6
  • Negative Genetic: 623
  • Phenotypic Enhancement: 8
  • Phenotypic Suppression: 6
  • Positive Genetic: 104
  • Synthetic Growth Defect: 26
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 65
  • Synthetic Rescue: 7
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.