ARB1 / YER036C Overview


Standard Name
ARB1 1
Systematic Name
YER036C
SGD ID
SGD:S000000838
Feature Type
ORF , Verified
Description
ATPase of the ATP-binding cassette (ABC) family; involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; important for disaggregation of ordered and disordered aggregates; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p; human homolog ABCF2 can complement yeast ARB1 mutant 1 2 3
Name Description
ATP-binding cassette protein involved in Ribosome Biogenesis 1
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
610
Mol. Weight (Da)
68370.8
Isoelectric Point
6.47
Median Abundance (molecules/cell)
32089 +/- 8268
Half-life (hr)
9.5

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all ARB1 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
ATPase involved in export of small ribosomal subunits from the nucleus and ribosome biogenesis; localizes in nucleus

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Null mutants are inviable under standard aerobic conditions, viable but slow-growing under anaerobic conditions; reduced function mutants show decreased competitive fitness
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


160 total interactions for 136 unique genes

Physical Interactions

  • Affinity Capture-MS: 127
  • Affinity Capture-RNA: 10
  • Affinity Capture-Western: 6
  • Biochemical Activity: 5
  • Co-localization: 1
  • Co-purification: 1
  • PCA: 1
  • Proximity Label-MS: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 1

Genetic Interactions

  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 3
  • Synthetic Rescue: 2
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
ARB1 encodes an ATPase from the ATP-binding cassette (ABC) superfamily that is involved in ribosome biogenesis. Arb1p appears to be required for several steps in the biogenesis of both 40S and 60S ribosomal subunits, including the processing of 18S and 25S rRNA precursors in the nucleus, and the nuclear export of pre-40S and pre-60S particles. Consistent with its role in ribosome maturation, Arb1p shuttles between nucleus and cytoplasm. Also consistently, ARB1 is among the targets of Sfp1p transcription factor that regulates expression of ribosomal protein and biogenesis genes. It is proposed that the ATP-hydrolyzing activity of Arb1p provides a mechanochemical force required at multiple steps of ribosome biogenesis. Yet the exact mechanisms of Arb1p actions remain to be discovered, as do pathways that regulate its activity.
Regulators
13
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
6
Additional
14
Reviews
9

Resources