Protein Help

ISC1 / YER019W Protein

Protein abundance data, domains, shared domains with other proteins, protein sequence retrieval for various strains, sequence-based physico-chemical properties, protein modification sites, and external identifiers for the protein.


Protein Product
inositol phosphosphingolipid phospholipase
Feature Type
ORF , Verified

AlphaFold Protein Structure

AlphaFold, developed by DeepMind, is an AI program that accurately predicts protein structures from amino acid sequences, enabling visualization of protein conformations. The predicted structures can be accessed through the Protein Data Bank (PDB) and AlphaFold Protein Structure Database.


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Model Confidence

Very high
Confident
Low
Very low

Experimental Data

Contains experimentally-derived protein half-life data obtained using stable isotope labeling by amino acids (SILAC) coupled with mass spectrometry. This section also contains protein abundance data for both untreated and treated cells obtained from over 20 studies. These data have been normalized and converted to a common unit of molecules per cell.


Protein Half Life

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ExperimentResultReference
half-life14.0 hrChristiano R, et al. (2014)

Protein Abundance

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Abundance (molecules/cell)MediaTreatmentTreatment timeFold ChangeVisualizationStrain backgroundOriginal ReferenceReference
955SDuntreatedquantitative mass spectrometry evidenceS288Cde Godoy LM, et al. (2008)Ho B, et al. (2018)
2170YEPDuntreatedqualitative western immunoblotting evidenceS288CGhaemmaghami S, et al. (2003)Ho B, et al. (2018)
279YEPDuntreatedquantitative mass spectrometry evidenceS288CKulak NA, et al. (2014)Ho B, et al. (2018)
1519F1untreatedquantitative mass spectrometry evidenceS288CLawless C, et al. (2016)Ho B, et al. (2018)
6704YEPDuntreatedquantitative mass spectrometry evidenceS288CLee MV, et al. (2011)Ho B, et al. (2018)
Showing 1 to 5 of 9 entries

Domains and Classification - S288C

Collection of computationally identified domains and motifs, as determined by InterProScan analysis; includes protein coordinates for the domain, a domain Description, a Source and corresponding accession ID, and the number of S. cerevisiae genes that share the same domain.


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Gene Protein Coordinates Accession ID Description Source No. of Genes with Domain

Domain Locations

Visual representation of the locations of the domains within the protein, as listed in the Domains and Classification table. Each row displays the domain(s) derived from a different Source, with domains color-coded according to this Source.

Scroll over a domain to view its exact coordinates and its Description.

Shared Domains

This diagram displays domains (colored squares) shared between the given protein (yellow circle) and other proteins (gray circles); the domains are color-coded according to their source, as displayed in the Domain Locations table, above.

Reset

Click on a gene or domain name to go to its specific page within SGD; drag any of the gene or domain objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram.

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.


isc1-K168A | isc1-supp1 | isc1-Δ

View all ISC1 alleles in SGD search

Sequence

Protein sequence for the given gene in S288C and other strains, when available. Use the pull-down menu under "Strain" to select the sequence for a specific strain. The displayed sequence can be downloaded in FASTA format as a .txt file. Amino acids displayed in blue represent modification sites. More detailed evidence for these modification sites is presented in the Post-translational Modifications table, located just below the protein sequence.


1 MYNRKDRDVH ERKEDGQSEF EALNGTNAIM SDNSKAYSIK FLTFNTWGLK YVSKHRKERL
61 RAIADKLAGH SMLTPISDEL LPNGGDSNEN EDYDVIALQE IWCVEDWKYL ASACASKYPY
121 QRLFHSGILT GPGLAILSKV PIESTFLYRF PINGRPSAVF RGDWYVGKSI AITVLNTGTR
181 PIAIMNSHMH APYAKQGDAA YLCHRSCQAW DFSRLIKLYR QAGYAVIVVG DLNSRPGSLP
241 HKFLTQEAGL VDSWEQLHGK QDLAVIARLS PLQQLLKGCT TCDSLLNTWR AQRQPDEACR
301 LDYALIDPDF LQTVDAGVRF TERIPHLDCS VSDHFAYSCT LNIVPQGTES RPSTSVKRAK
361 THDRELILQR YSNYETMIEC IHTYLKTAQR QKFFRGLHFW ASILLLIASL VVTTFTANKA
421 GWSSIFWVLF AIAVSISGTI DGAISFLFGR SEIRALIEVE QEVLDAEHHL QTFLSEK*

* Blue amino acids indicate modification sites. More information below.

Post-translational Modifications - S288C

Modification sites for the protein in the selected strain, based on the presence of a residue in the specific strain, as inferred from experimental evidence.

5 entries for 3 sites

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SiteModificationModifierReference
K117ubiquitinylated lysineSwaney DL, et al. (2013) PMID: 23749301
S270phosphorylated residueLanz MC, et al. (2021) PMID: 33491328
T361phosphorylated residueSchmitt K, et al. (2017) PMID: 27821475
T361phosphorylated residueLanz MC, et al. (2021) PMID: 33491328
T361phosphorylated residueLeutert M, et al. (2023) PMID: 37845410
Showing 1 to 5 of 5 entries

Sequence-Based Physico-chemical Properties - S288C

Calculated protein properties, including amino acid composition, length, coding region calculations, and atomic composition.

Amino Acid Composition

Sort table using the arrow to the right of a column header to sort by that column; download all properties as a .txt file using the "Download Properties" button.

Amino AcidFrequencyPercentage
A408.39
C102.10
D275.66
E255.24
F214.40
G275.66
H163.35
I326.71
K234.82
L5010.48
M61.26
N163.35
P173.56
Q204.19
R296.08
S398.18
T275.66
V245.03
W102.10
Y183.77

Physical Details

Length (a.a): 477
Molecular Weight (Da): 53941.1
Isoelectric Point (pl): 7.58
Formula: C2422H3761N665O702S16
Aliphatic Index: 85.15
Instability Index: 43.42

Coding Region Translation Calculations

Codon Bias: 0.2
Codon Adaptation Index: 0.13
Frequence of Optimal Codons: 0.54
Hydropathicity of Protein: -0.21
Aromaticity Score: 0.1

Extinction Coefficients at 280nm

ALL Cys residues appear as half cystines: 82445.0
NO Cys residues appear as half cystines: 81820.0

Atomic Composition

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Atom Frequency Percentage

Data not found or not available for  S288C

External Identifiers

List of external identifiers for the protein from various database sources.

15 entries for 8 sources


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External IDSource
orf19.6629CGD
5002DIP
AAB64552.1GenBank/EMBL/DDBJ
U18778GenBank/EMBL/DDBJ
731443GenBank/EMBL/DDBJ
603611GenBank/EMBL/DDBJ
6320856GenBank/EMBL/DDBJ
NM_001178910.1NCBI
856739NCBI
DAA07671.1NCBI
Showing 1 to 10 of 15 entries

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