NPR2 / YEL062W Overview


Standard Name
NPR2 1
Systematic Name
YEL062W
SGD ID
SGD:S000000788
Feature Type
ORF , Verified
Description
Subunit of the Iml1p/SEACIT complex; SEACIT (Iml1p-Npr2p-Npr3p) is a subcomplex of the SEA complex, a coatomer-related complex that associates dynamically with the vacuole; Npr2p may have a structural or regulatory role, supporting Iml1p function as a GAP for the Rag family GTPase Gtr1p, and resulting in inhibition of TORC1 signaling in response to amino acid deprivation; SEACIT is required for non-nitrogen-starvation-induced autophagy; homolog of human tumor suppressor NPRL2 1 2 3 4 5 6 7 8
Name Description
Nitrogen Permease Regulator 1
Comparative Info
Integrated model organism details available at the Alliance of Genome Resources website
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
615
Mol. Weight (Da)
69868.4
Isoelectric Point
8.69
Median Abundance (molecules/cell)
1643 +/- 727
Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Subunit of SEACIT, a subcomplex of the SEA complex that acts as a GTPase-activating protein (GAP) to negatively regulates signaling through the heterodimeric Gtr1p-Gtr2p GTPase; negative regulator of TORC1 signaling in response to amino acid deprivation; positive regulator of autophagy and autophagosome formation; loosely associated with the vacuolar membrane

View computational annotations

Molecular Function

Manually Curated

Complex

Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.


Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null mutant shows abnormal localization of Tor1p and increased phosphorylation of Sch9p; null mutation results in defective autophagy and lower utilization of proline, ammonium or urea as nitrogen source; null mutant is resistant to cisplatin and doxorubicin; homozygous null mutant diploid has defects in meiosis and sporulation; in systematic studies null mutant is sensitive to azole drugs, tunicamycin, DTT, camptothecin, but resistant to bleomycin and caffeine
Disease Details

Disease

Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.


Summary
Yeast NPR2 is homologous to human DEPDC5, and has been used to study cancer

Manually Curated

Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


178 total interactions for 146 unique genes

Physical Interactions

  • Affinity Capture-MS: 20
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 16
  • Two-hybrid: 2

Genetic Interactions

  • Dosage Lethality: 1
  • Negative Genetic: 99
  • Phenotypic Suppression: 8
  • Positive Genetic: 15
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 8
  • Synthetic Rescue: 1
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Regulators
6
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
26
Additional
17
Reviews
8

Resources