Phenotype Help

WBP1 / YEL002C Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.


Summary
Essential gene; ts mutant displays under-glycosylation of carboxypeptidase Y at the permissive temperature and increased apoptosis at restrictive temperature; mutant is sensitive to hygromycin B; in large-scale studies, repression confers decreased competitive fitness, increased frequency of chromosomal rearrangements and loss, and sensitivity to MMS

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

19 entries for 11 phenotypes


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

PhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetailsReference
apoptosis: increased
classical geneticsconditional
Allele: wbp1-1
OtherTemperature: elevated temperature, 37 °C
Details: detected by DAPI and Annexin V-FITC staining and TUNEL assay
Hauptmann P, et al. (2006) PMID:16420350
chromosome/plasmid maintenance: decreased
large-scale surveyrepressibleS288C10 ug/ml doxycyclineDetails: Tet-regulated essential ORF strain collection (doxycycline represses expression)|; increased chromosome rearrangements and chromosome loss, as measured in an illegitimate mating assay
Cheng E, et al. (2012) PMID:22673806
competitive fitness: decreased
competitive growth

fitness profiling of essential genes using hypomorphic DAmP alleles

reduction of functionS288CMedia: minimal medium
Details: Relative fitness score: 0.991
Breslow DK, et al. (2008) PMID:18622397
haploinsufficient
heterozygous diploid, systematic mutation set null
Allele: wbp1-Δ
S288COhnuki S and Ohya Y (2018) PMID:29768403
heat sensitivity: increased
classical geneticsconditional
Allele: wbp1-1
X2180-1ATemperature: elevated temperature, 37 °C
te Heesen S, et al. (1992) PMID:1600939
inviable
systematic mutation setnull
Allele: wbp1-Δ
S288CGiaever G, et al. (2002) PMID:12140549
protein/peptide modification: decreased
Reporter: Prc1p
classical geneticsrepressibleX2180-1ADetails: carboxypeptidase Y is not fully glycosylated
te Heesen S, et al. (1992) PMID:1600939
protein/peptide modification: decreased
Reporter: Prc1p
classical geneticsconditional
Allele: wbp1-1
X2180-1ATemperature: permissive temperature, 23 °C
Details: carboxypeptidase Y is not fully glycosylated
te Heesen S, et al. (1992) PMID:1600939
resistance to chemicals: decreased
classical geneticsunspecifiedOther50 ug/ml hygromycin BDean N (1995) PMID:7877969
resistance to chemicals: decreased
systematic mutation set

Decreased Abundance by mRNA Perturbation (DAmP) library of haploid strains

reduction of functionS288C methyl methanesulfonateSvensson JP, et al. (2011) PMID:21978764
Showing 1 to 10 of 19 entries

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).


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