Phenotype Help

CDC39 / YCR093W Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.


Summary
Essential gene in reference strain S288C; conditional mutant arrests in the G1 phase at START, as conjugation-competent, unbudded cells with single, undivided nuclei and pear-shaped morphology; conditional mutant displays elevated colony sectoring indicative of chromosomal instability and increased nucleolar size; homozygous diploid transposon insertion mutant is unable to undergo nitrogen-starvation induced pseudohyphal growth; heterozygous diploid null mutant has increased innate thermotolerance; overexpression results in an accumulation of cells with a 2C DNA content and an increased percentage of large-budded cells indicative of a G2 or M phase delay or arrest

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

26 entries for 17 phenotypes


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

PhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetailsReference
cell cycle passage through START: arrested
classical geneticsconditional
Allele: cdc39-1
OtherTemperature: elevated temperature, 37 °C
Details: arrest as conjugation-competent, unbudded cells with single, undivided nuclei and pear-shaped morphology
Reed SI (1980) PMID:7002718
cell cycle progression: abnormal
systematic mutation setoverexpressionS288CDetails: accumulation of cells with a 2C DNA content and an increased percentage of large-budded cells indicative of a G2 or M phase delay or arrest
Niu W, et al. (2008) PMID:18617996
cell shape: abnormal
classical geneticsconditional
Allele: cdc39-1
OtherTemperature: elevated temperature, 37 °C
Details: pear-shaped morphology
Reed SI (1980) PMID:7002718
colony sectoring: increased
large-scale surveyconditional
Allele: cdc39-1
S288CAssay: chromosome transmission fidelity (CTF) assay
Temperature: semi-permissive temperature
Details: indicative of chromosome instability (CIN); metascore weak (single hit)
Stirling PC, et al. (2011) PMID:21552543
competitive fitness: increased
competitive growth

fitness profiling of essential genes using hypomorphic DAmP alleles

reduction of functionS288CMedia: minimal medium
Details: Relative fitness score: 1.003
Breslow DK, et al. (2008) PMID:18622397
heat sensitivity: increased
classical geneticsconditional
Allele: cdc39-1
OtherTemperature: elevated temperature, 37 °C
Reed SI (1980) PMID:7002718
innate thermotolerance: increased
heterozygous diploid, systematic mutation setnull
Allele: cdc39-Δ
S288CPhase: exponential phase
Treatment: heat shock, 50 °C
Details: resistance scale of 1 (least) to 4 (most) resistant: 3
Jarolim S, et al. (2013) PMID:24142923
inviable
systematic mutation setnull
Allele: cdc39-Δ
S288CGiaever G, et al. (2002) PMID:12140549
metal resistance: decreased
systematic mutation setnull
Allele: cdc39-Δ
S288C60 nM methylmercury chlorideHwang GW, et al. (2014) PMID:25074250
protein/peptide accumulation: increased
Reporter: R-Ura3, UbV76-eK-Ura3, DHFRmutC, ODS-Ura3

ubiquitin proteasome system (UPS) substrates

classical genetics null
Allele: cdc39-Δ
S288CKandasamy G, et al. (2021) PMID:33727038
Showing 1 to 10 of 26 entries

Shared Phenotypes

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