Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.
A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.
13 entries for 8 phenotypesIncrease the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.
| Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details | Reference |
|---|---|---|---|---|---|---|
| competitive fitness: normal | competitive growth fitness profiling of essential genes using hypomorphic DAmP alleles | reduction of function | S288C | Media: minimal medium Details: Relative fitness score: 1.000 | Breslow DK, et al. (2008) PMID:18622397 | |
| haploinsufficient | heterozygous diploid, systematic mutation set | null Allele: rsa4-Δ | S288C | Ohnuki S and Ohya Y (2018) PMID:29768403 | ||
| heat sensitivity: increased | large-scale survey | conditional Allele: rsa4-ts | S288C | Temperature: elevated temperature, 37 °C Details: very slow growth | Ben-Aroya S, et al. (2008) PMID:18439903 | |
| inviable | systematic mutation set | null Allele: rsa4-Δ | S288C | Giaever G, et al. (2002) PMID:12140549 | ||
| inviable | classical genetics | dominant negative Allele: rsa4-E114D N-terminal ubiquitin like domain, MIDAS ion coordination site mutated | W303 | Ulbrich C, et al. (2009) PMID:19737519 | ||
| inviable | classical genetics | unspecified Allele: rsa4-E114A N-terminal ubiquitin like domain, MIDAS ion coordination site mutated | W303 | Ulbrich C, et al. (2009) PMID:19737519 | ||
| inviable | classical genetics | overexpression Allele: rsa4-E114D N-terminal ubiquitin like domain, MIDAS ion coordination site mutated; dominant negative | W303 | Ulbrich C, et al. (2009) PMID:19737519 | ||
| protein/peptide accumulation: decreased Reporter: 60S ribosomal subunit | classical genetics | overexpression Allele: rsa4-E114D N-terminal ubiquitin like domain, MIDAS ion coordination site mutated; dominant negative | W303 | Details: reduction in mature 60S ribosomal subunit, relative to 40S and the appearance of half-mer polysomes | Ulbrich C, et al. (2009) PMID:19737519 | |
| protein/peptide distribution: abnormal Reporter: pre-60S ribosomal subunit | classical genetics | overexpression Allele: rsa4-E114D N-terminal ubiquitin like domain, MIDAS ion coordination site mutated; dominant negative | W303 | Details: accumulation of pre-60S ribosomal subunits in the nucleoplasm | Ulbrich C, et al. (2009) PMID:19737519 | |
| resistance to chemicals: decreased | systematic mutation set | conditional Allele: rsa4-5001 | S288C | 2 ug/mL camptothecin | Forster DT, et al. (2022) PMID:36192463 |
This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).
Click on a gene or phenotype observable name to go to its specific page within SGD; drag any of the gene or observable objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram; filter the genes that share observable terms with the given gene by the number of terms they share by clicking anywhere on the slider bar or dragging the tab to the desired filter number.
Addgene Plasmids | DNASU Plasmids | PlasmID | Thermo Scientific | YGRC
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