SCO1 / YBR037C Overview
- Standard Name
- SCO1 1
- Systematic Name
- YBR037C
- SGD ID
- SGD:S000000241
- Aliases
- PET161
- Feature Type
- ORF , Verified
- Description
- Copper-binding protein of mitochondrial inner membrane; required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; similar to thioredoxins; SCO1 has a paralog, SCO2, that arose from the whole genome duplication 2 3 4 6
- Name Description
- Suppressor of Cytochrome Oxidase deficiency 5
- Paralog
- SCO2 6
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- SCO1 has a paralog, SCO2, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 295
- Mol. Weight (Da)
- 33174.3
- Isoelectric Point
- 9.46
- Median Abundance (molecules/cell)
- 2467 +/- 767
- Half-life (hr)
- 35.8
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all SCO1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Putative thioredoxin peroxidase involved in protein complex assembly and copper ion transport; likely involved in response to redox state; localizes to mitochondrial inner membrane
View computational annotations
Molecular Function
- Manually Curated
- enables copper ion binding (IDA)
Biological Process
- Manually Curated
- involved in cell redox homeostasis (IGI)
- involved in cellular response to oxidative stress (IGI)
- involved in mitochondrial cytochrome c oxidase assembly (IMP)
Cellular Component
- Manually Curated
- located in mitochondrial inner membrane (IDA)
- located in mitochondrion (HDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant and sco1-C148A mutant with defective copper binding site are unable to utilize nonfermentable carbon sources; null mutant is sensitive to oxidative stress but sco1-C148A mutant is not; in large-scale studies, null mutant shows lower competitive fitness in several different media, smaller cell size, shorter chronological lifespan, and sensitivity to desiccation and hyperosmotic stress; diploid homozygous null mutant accumulates reduced levels of glycogen and excretes higher levels of glutathione; null mutant has altered resistance to a variety of chemicals
Classical Genetics
- null
- cell size: decreased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chemical compound excretion: increased
- chronological lifespan: decreased
- competitive fitness: decreased
- desiccation resistance: decreased
- haploproficient
- hyperosmotic stress resistance: decreased
- mitotic recombination: increased
- oxidative stress resistance: decreased
- oxidative stress resistance: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: decreased
- respiratory growth: absent
- respiratory growth: decreased rate
- sporulation efficiency: decreased
- utilization of carbon source: decreased rate
- vegetative growth: decreased rate
- viable
- overexpression
- unspecified
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast SCO1 is homologous to human SCO1, and has been used to study neonatal hepatopathy and hypertrophic cardiomyopathy
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The sco1 null mutant is viable; the null mutant of paralog sco2 is viable; the sco1 sco2 double mutant has not been annotated for phenotype.
212 total interactions for 173 unique genes
Physical Interactions
- Affinity Capture-MS: 5
- Affinity Capture-RNA: 7
- Affinity Capture-Western: 4
- Biochemical Activity: 1
- Co-fractionation: 1
- Co-localization: 1
- Co-purification: 2
- PCA: 1
- Proximity Label-MS: 7
- Reconstituted Complex: 2
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Rescue: 9
- Negative Genetic: 127
- Phenotypic Enhancement: 1
- Phenotypic Suppression: 1
- Positive Genetic: 35
- Synthetic Growth Defect: 4
- Synthetic Lethality: 2
- Synthetic Rescue: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 4
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.