EDE1 / YBL047C Overview

Standard Name
EDE1 1
Systematic Name
BUD15 8
Feature Type
ORF , Verified
Endocytic adaptor; K63-specific Ub-chain binding protein involved in receptor endocytosis; scaffold protein that localizes to nascent endocytic sites after homo-oligomerization and organizes the endocytic machinery; role in autophagy of aberrant clathrin-mediated endocytosis protein condensates; binds membranes in a Ub-dependent manner; interacts with and functions upstream of Cmk2p to suppress stress-induced non-apoptotic cell death; putative cytokinesis regulator; homolog of mammalian Eps15 1 2 3 4 5 6 7
Name Description
EH Domains and Endocytosis 1
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

EDE1 is located on the left arm of chromosome II between uncharacterized gene RRT1 and protein phosphatase PP4 subunit PSY4; coding sequence is 4146 nucleotides long with 39 SNPs, 9 of which cause amino acid polymorphisms
Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Ede1p is 1381 amino acids long, longer-lived, moderate in abundance; contains 3 EH domains, 2 EF-hand domains, and 1 ubiquitin-associated domain
Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)
12983 +/- 3892
Half-life (hr)


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.

View all EDE1 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Involved in organization of endocytic sites and in the regulation of cytokinesis; binds ubiquitin; localizes to actin cortical patches, bud neck, bud tip, cytoplasm, and shmoo tip

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated
Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Non-essential gene; null mutant displays small defect in vacuolar fragmentation, defective endocytosis, abnormal cell shape, sensitivity to visible light, increased resistance to hydroxyurea and to killer toxins; null is sensitive to bleomycin, fluconazole, fenpropimorph, caffeine, rapamycin, Calcofluor White, mercaptoethanol; overexpression confers resistance to ethanol
Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

Ede1p interacts physically with proteins involved in cytoskeleton organization; EDE1 interacts genetically with genes involved in protein targeting

584 total interactions for 372 unique genes

Physical Interactions

  • Affinity Capture-MS: 42
  • Affinity Capture-RNA: 10
  • Affinity Capture-Western: 8
  • Biochemical Activity: 9
  • Co-crystal Structure: 2
  • Co-localization: 3
  • FRET: 1
  • PCA: 3
  • Protein-RNA: 1
  • Reconstituted Complex: 11
  • Two-hybrid: 18

Genetic Interactions

  • Dosage Growth Defect: 3
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 279
  • Phenotypic Enhancement: 115
  • Phenotypic Suppression: 12
  • Positive Genetic: 34
  • Synthetic Growth Defect: 18
  • Synthetic Lethality: 12
  • Synthetic Rescue: 1
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

EDE1 promoter is bound by Reb1p, and by Sua7p and Xbp1p in response to heat; Ede1 protein activity is regulated by Cdc28p and protein phosphatase 2A
Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.

Last Updated: 2004-03-28

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.