PET9 / YBL030C Overview


Standard Name
PET9 1
Systematic Name
YBL030C
SGD ID
SGD:S000000126
Aliases
AAC2 13 , ANC2 22 , OP1 23
Feature Type
ORF , Verified
Description
Major ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; also functions in peroxisomes; required for viability in many lab strains that carry a sal1 mutation; PET9 has a paralog, AAC3, that arose from the whole genome duplication; human homolog SLC25A4 implicated in progressive external ophthalmoplegia can complement yeast null mutant 2 3 4 5 6 7 8 10
Name Description
PETite 9
Paralog
AAC3 7
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
PET9 has a paralog, AAC3, that arose from the whole genome duplication
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
318
Mol. Weight (Da)
34446.7
Isoelectric Point
10.33
Median Abundance (molecules/cell)
29722 +/- 8755
Half-life (hr)
6.8

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.


View all PET9 alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
ATP/ADP exchanger of the mitochondrial inner membrane involved in respiration and apoptosis

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene in W303, essential gene in other strain backgrounds; null mutants grow slowly, cannot respire, are sensitive to heat and cold; missense mutants that correspond to human disease mutations display electron transport chain damage, hypersensitivity to the chemical uncoupler CCCP, mtDNA instability, and intolerance to mtDNA loss; overexpression slows growth
Disease Details

Disease

Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.


Summary
Yeast PET9 is homologous to human SLC25A4, and has been used to study mitochondrial myopathy, lactic acidosis, facioscapulohumeral muscular dystrophy, hypertrophic cardiomyopathy, Sengers syndrome, and autosomal dominant progressive external ophthalmoplegia with mitochondrial DNA deletions
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
The pet9 null mutant is inviable in the S288C background, but viable in W303; the null mutant of paralog aac3 is viable; the pet9 aac3 double mutant displays a synthetic growth defect.

370 total interactions for 268 unique genes

Physical Interactions

  • Affinity Capture-MS: 155
  • Affinity Capture-RNA: 10
  • Affinity Capture-Western: 41
  • Biochemical Activity: 1
  • Co-purification: 1
  • Cross-Linking-MS (XL-MS): 19
  • PCA: 3
  • Proximity Label-MS: 4
  • Reconstituted Complex: 1
  • Two-hybrid: 1

Genetic Interactions

  • Dosage Growth Defect: 1
  • Dosage Lethality: 2
  • Dosage Rescue: 49
  • Negative Genetic: 20
  • Phenotypic Enhancement: 3
  • Positive Genetic: 22
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 21
  • Synthetic Rescue: 12
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Regulators
17
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.


Last Updated: 2010-05-10

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
122
Additional
97
Reviews
38

Resources