Phenotype Help

RFT1 / YBL020W Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.


Summary
Essential gene; depletion results in accumulation of dolichol-bound Man5GlcNAc2 on the cytoplasmic face of ER membrane, underglycosylation and defective secretion of proteins, and opi- phenotype (excretion of inositol in the absence of inositol and choline); overexpression leads to slow growth

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

15 entries for 10 phenotypes


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

PhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetailsReference
chemical compound accumulation: increased
classical geneticsrepressibleOther alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-D-GlcNAc(PP-Dol)Rush JS, et al. (2009) PMID:19494107
chemical compound excretion: increased
systematic mutation set

Dox titratable strains: Tet-regulated promoter that is shut off by the addition of doxycycline

repressibleS288C inositolDetails: Opi- phenotype; overproduction and excretion of inositol in the absence of inositol and choline
Salas-Santiago B and Lopes JM (2014) PMID:24558266
colony appearance: abnormal
systematic mutation setnull
Allele: rft1-Δ
Sigma1278bMedia: YP sucrose plates
Details: semi-smooth morphology compared to the fully wrinkled appearance of wt colonies
Voordeckers K, et al. (2012) PMID:22882838
competitive fitness: increased
competitive growth

fitness profiling of essential genes using hypomorphic DAmP alleles

reduction of functionS288CMedia: minimal medium
Details: Relative fitness score: 1.007
Breslow DK, et al. (2008) PMID:18622397
inviable
systematic mutation setnull
Allele: rft1-Δ
S288CGiaever G, et al. (2002) PMID:12140549
protein transport: abnormal
systematic mutation setrepressibleS288CDetails: Kar2p is secreted rather than retained in the ER
Copic A, et al. (2009) PMID:19433630
protein/peptide modification: decreased
Reporter: carboxypeptidase Y
classical geneticsrepressibleOtherDetails: carboxypeptidase Y is underglycosylated
Helenius J, et al. (2002) PMID:11807558
protein/peptide modification: decreased
Reporter: carboxypeptidase Y (CPY)
classical genetics repressible
Allele: rft1-Δ
W303Details: decrease in the addition of N-linked glycans to CPY resulting the characteristic ladder of CPY intermediates
Chen S, et al. (2024) PMID:38886340
resistance to chemicals: decreased
heterozygous diploid, systematic mutation set null
Allele: rft1-Δ
S288C312.5 μg/mL imipramineLum PY, et al. (2004) PMID:14718172
resistance to chemicals: decreased
heterozygous diploid, systematic mutation set null
Allele: rft1-Δ
S288C150 ng/mL tunicamycinLum PY, et al. (2004) PMID:14718172
Showing 1 to 10 of 15 entries

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).


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Click on a gene or phenotype observable name to go to its specific page within SGD; drag any of the gene or observable objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram; filter the genes that share observable terms with the given gene by the number of terms they share by clicking anywhere on the slider bar or dragging the tab to the desired filter number.


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