OAF1 / YAL051W Overview
- Standard Name
- OAF1 1
- Systematic Name
- YAL051W
- SGD ID
- SGD:S000000048
- Aliases
- YAF1
- Feature Type
- ORF , Verified
- Description
- Oleate-activated transcription factor; subunit of a heterodimeric complex with Pip2p, which binds to oleate-response elements (ORE) in the promoter of genes involved in beta-oxidation of fatty acids, peroxisome organization and biogenesis, activating transcription in the presence of oleate; regulates chromatin silencing at telomeres; involved in diauxic shift; OAF1 has a paralog, PIP2, that arose from the whole genome duplication 2 3 4 5 6 7 8
- Name Description
- Oleate-Activated transcription Factor 1
- Paralog
- PIP2 4
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- OAF1 is located on the left arm of chromosome I between FLC2 putative calcium channel and AIM2 cytoplasmic protein; coding sequence is 3144 nucleotides long with 7 nonsynonymous SNPs, 24 synonymous SNPs; OAF1 has paralog PIP2 from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 1047
- Mol. Weight (Da)
- 118886.4
- Isoelectric Point
- 6.98
- Median Abundance (molecules/cell)
- 744 +/- 684
- Half-life (hr)
- 1.4
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.
View all OAF1 alleles in SGD search | YeastMine
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Subunit of the heterodimeric Pip2-Oaf1 complex, which binds to promoter proximal elements upstream of genes involved in peroxisomal proliferation, functioning as a transcriptional activator in the presence of oleate; regulates chromatin silencing at telomeres
View computational annotations
Molecular Function
- Manually Curated
- enables DNA-binding transcription activator activity, RNA polymerase II-specific (IMP, IDA)
- contributes to RNA polymerase II cis-regulatory region sequence-specific DNA binding (IMP, IDA)
- enables sequence-specific DNA binding (HDA)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in cellular response to oleic acid (IMP)
- involved in positive regulation of fatty acid beta-oxidation (IMP)
- involved in positive regulation of transcription by RNA polymerase II (IMP)
- involved in positive regulation of transcription from RNA polymerase II promoter by oleic acid (IMP, IGI)
Cellular Component
- Manually Curated
- part of Pip2-Oaf1 complex (IPI, IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
Classical Genetics
- null
- auxotrophy
- chronological lifespan: increased
- competitive fitness: decreased
- competitive fitness: increased
- heat sensitivity: increased
- metal resistance: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- stress resistance: decreased
- utilization of nitrogen source: decreased rate
- vacuolar morphology: abnormal
- viable
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The oaf1 null mutant is viable; the null mutant of paralog pip2 is viable; the oaf1 pip2 double mutant has not been annotated for phenotype.
103 total interactions for 87 unique genes
Physical Interactions
- Affinity Capture-MS: 11
- Affinity Capture-RNA: 5
- Affinity Capture-Western: 2
- Biochemical Activity: 1
- Co-localization: 4
- Reconstituted Complex: 3
- Two-hybrid: 2
Genetic Interactions
- Negative Genetic: 45
- Phenotypic Suppression: 2
- Positive Genetic: 22
- Synthetic Growth Defect: 5
- Synthetic Rescue: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- OAF1 encodes a transcription factor that is a member of the C6 zinc finger class, containing a DNA binding domain also known as the Zn2Cys6 binuclear zinc cluster or zinc knuckle. Acting primarily as a heterodimer with its paralog Pip2p, Oaf1p binds to oleate response elements in the promoters of genes involved in fatty acid beta-oxidation and peroxisome biogenesis, such as POX1, POT1, and CTA1. The heterodimer binds constitutively to promoters, and is activated by binding of oleate to Oaf1p. The Oaf1p-Pip2p heterodimer also activates PIP2 transcription. Independently of Pip2p, Oaf1p binds to subtelomeric regions and may promote silencing in response to fatty acids. Although OAF1 transcription is constitutive, Oaf1p activity is regulated: in addition to being activated by oleate, Oaf1p is activated by phosphorylation under inducing conditions.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.