RBG1 / YAL036C Overview


Standard Name
RBG1 1 2
Systematic Name
YAL036C
SGD ID
SGD:S000000034
Aliases
FUN11 7
Feature Type
ORF , Verified
Description
Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid; localizes to cytosol and peroxisomes 2 3 4 5 6
Name Description
RiBosome interacting Gtpase 1
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
RBG1 is located on the left arm of chromosome I between uncharacterized gene YAL037W and FUN12 translation initiation factor eIF5B; coding sequence is 1110 nucleotides long with 17 synonymous SNPs
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Rbg1p is 369 amino acids long, somewhat short-lived, low-to-moderate in abundance; contains 4 GTP binding domains; phosphorylated on 3 residues, ubiquitinylated on 5 lysines
Length (a.a.)
369
Mol. Weight (Da)
40704.5
Isoelectric Point
8.57
Median Abundance (molecules/cell)
8422 +/- 2284
Half-life (hr)
6.3

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.


View all RBG1 alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Cytoplasmic GTP-binding protein involved in translation and up-regulation of amino acid starvation response; localizes to polysomes and stress granules

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null mutant displays increased competitive fitness, decreased resistance to visible light stress, sensitivity to antibiotics, and increased lifespan
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
Rbg1p interacts physically with proteins involved in translation; RBG1 interacts genetically with genes involved in transcription and organelle organization

212 total interactions for 176 unique genes

Physical Interactions

  • Affinity Capture-MS: 106
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 11
  • Co-crystal Structure: 1
  • Co-fractionation: 2
  • Co-purification: 1
  • Proximity Label-MS: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 9

Genetic Interactions

  • Dosage Growth Defect: 1
  • Negative Genetic: 52
  • Phenotypic Enhancement: 1
  • Positive Genetic: 12
  • Synthetic Growth Defect: 9
  • Synthetic Rescue: 1
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
RBG1 promoter is bound by Med2p and Rfx1p in response to heat; RBG1 transcription is regulated by Gcr1p, Sfp1p, and Spt10p
Regulators
7
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
13
Additional
7
Reviews
2

Resources