RDS2 / YPL133C Overview


Standard Name
RDS2 1
Systematic Name
YPL133C
SGD ID
SGD:S000006054
Feature Type
ORF , Verified
Description
Transcription factor involved in regulating gluconeogenesis; also involved in the regulation of glyoxylate cycle genes; member of the zinc cluster family of proteins; confers resistance to ketoconazole 1 2 3
Name Description
Regulator of Drug Sensitivity 1
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
446
Mol. Weight (Da)
50084.3
Isoelectric Point
7.78
Median Abundance (molecules/cell)
799 +/- 587
Half-life (hr)
5.3

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all RDS2 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
DNA and zinc binding protein; binds RNA polymerase II cis-regulatory regions of DNA; positively regulates transcription; involved in cellular response to xenobiotic stimulus; localizes to the nucleus

View computational annotations

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


65 total interactions for 55 unique genes

Physical Interactions

  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 1
  • Biochemical Activity: 11
  • Reconstituted Complex: 12
  • Two-hybrid: 6

Genetic Interactions

  • Dosage Lethality: 1
  • Negative Genetic: 20
  • Phenotypic Enhancement: 1
  • Positive Genetic: 3
  • Synthetic Growth Defect: 1
  • Synthetic Haploinsufficiency: 2
  • Synthetic Rescue: 1
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
RDS2 encodes a transcription factor that is a member of the C6 zinc finger class, containing a DNA binding domain also known as the Zn2Cys6 binuclear zinc cluster or zinc knuckle. After a shift from glucose to ethanol or glycerol carbon source, Rds2p binds to carbon source response elements (CSREs) in the promoters of its target genes. Rds2p activates transcription of genes involved in gluconeogenesis, the TCA cycle, and respiration, and its targets also include some genes involved in resistance to stress and drugs. While Rds2p is primarily a positive regulator, it has been found to negatively regulate the expression of some genes encoding negative regulators of gluconeogenesis, such as PFK27 and GID8. Along with Rds2p, the transcription factors Cat8p and Sip4p also contribute to regulation of genes involved in gluconeogenesis. All three factors bind to target sites with the same consensus sequence, although each has a preference for particular variants of the sequence, and they have overlapping regulatory roles. The set of target genes regulated by Rds2p also overlaps with the set of genes regulated by Adr1p. Under inducing conditions, Rds2p is phosphorylated, primarily by the Snf1p kinase. Its phosphorylation is crucial for full activity at some promoters (for example, PKC1), but not others (for example, FBP1).
Regulators
4
Targets
11
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
13
Additional
16
Reviews
7

Resources