PUP1 / YOR157C Overview


Standard Name
PUP1 1
Systematic Name
YOR157C
SGD ID
SGD:S000005683
Feature Type
ORF , Verified
Description
Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z 2 3 4
Name Description
PUtative Proteasome subunit 1
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
PUP1 is on the right arm of chromosome XV between NFI1 SUMO E3 ligase and PET123 mitochondrial ribosomal protein; coding sequence is 786 nucleotides long with 4 SNPs, only 1 of which causes an amino acid polymorphism at residue 227
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Pup1p is 261 amino acids long, very short-lived, and high in abundance; contains 3 peptidase T1A proteasome beta-subunit domains; phosphorylated on 3 residues, ubiquitinylated on K58
Length (a.a.)
261
Mol. Weight (Da)
28264.2
Isoelectric Point
6.6
Median Abundance (molecules/cell)
11679 +/- 5298
Half-life (hr)
1.6

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all PUP1 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Endopeptidase subunit of 26S Proteasome complex; involved in proteasome-mediated protein catabolism; localized to ER membrane and proteasome storage granule

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated

Complex

Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.


Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Essential gene, heterozygous null is haploinsufficient and heat-sensitive; non-catalytic alleles impart cold-sensitivity and increased mutation frequency
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
Pup1p interacts physically with proteins involved in protein catabolism; PUP1 interacts genetically with genes involved in protein catabolism

486 total interactions for 300 unique genes

Physical Interactions

  • Affinity Capture-MS: 87
  • Affinity Capture-RNA: 6
  • Affinity Capture-Western: 21
  • Co-crystal Structure: 5
  • Co-fractionation: 1
  • Co-purification: 4
  • PCA: 1
  • Protein-peptide: 1
  • Proximity Label-MS: 1
  • Two-hybrid: 6

Genetic Interactions

  • Negative Genetic: 264
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 2
  • Positive Genetic: 75
  • Synthetic Growth Defect: 5
  • Synthetic Haploinsufficiency: 2
  • Synthetic Lethality: 2
  • Synthetic Rescue: 1
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
PUP1 promoter is bound by Fkh1p, Reb1p, Rpn4p; PUP1 promoter bound by Rfx1p, Tfc7p, and Xbp1p in response to heat; transcription of PUP1 regulated by Sfp1p in response to stress
Regulators
7
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.


Last Updated: 2010-06-29

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
37
Additional
96
Reviews
18

Resources