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| Dataset | Description | Keywords | Number of Conditions | Reference |
|---|---|---|---|---|
| A novel response to microtubule perturbation in meiosis | Cells were grown to saturation in YPD (YEP + 2% glucose) for 24 hours, diluted into YPA (YEP + 2% potassium acetate) at OD600= 0.3 and grown over night at 30C | sporulation, cytoskeleton and molecular motors | 26 | Hochwagen A, et al. (2005) PMID:15899877 |
| Comparison of Transcriptomes of Wild Type and aan1∆ cells by Next Generation RNA Sequencing | We use RNAseq to compare the transcriptomes of wild type and aan1∆ cells to determine the mechanism behind AAN1 function in the actin cytoskeleton and lifespan. We find deleting AAN1 results in changes in expression of genes involved in branched-chain amino acid synthesis. | cytoskeleton and molecular motors, cell aging | 2 | Sing CN, et al. (2022) PMID:35577788 |
| Distinct functional constraints driving conservation of the cofilin N-terminal regulatory tail | Cofilin family proteins have essential roles in remodeling the cytoskeleton through filamentous actin depolymerization and severing. The short unstructured N-terminal region of cofilin is critical for actin binding and harbors the major site of inhibitory phosphorylation. Atypically for a disordered sequence, the N-terminal region is highly conserved, but specifics aspects driving this conservation are unclear. Here, we screened a library of 16,000 human cofilin N-terminal sequence variants for their capacity to support growth in S. cerevisiae in the presence or absence of the upstream regulator LIM kinase. Results from the screen and biochemical analysis of individual variants revealed distinct sequence requirements for actin binding and regulation by LIM kinase. LIM kinase recognition only partly explained sequence constraints on phosphoregulation, which were instead driven to a large extent by the capacity for phosphorylation to inactivate cofilin. We found remarkably loose sequence requirements for actin binding and phosphoinhibition, but collectively they restricted the N-terminus to sequences found in natural cofilins. Our results illustrate how a phosphorylation site can balance potentially competing sequence requirements for function and regulation. | cytoskeleton and molecular motors | 14 | |
| Effects of antimitotic drug benomyl on wild-type strain and strains with Yap1 or Pdr1 deletions | Time-course analyses of the modifications of the yeast gene expression program which immediately follows addition of the antimitotic drug benomyl | chemical stimulus, mitotic cell cycle, cytoskeleton and molecular motors | 16 | Lucau-Danila A, et al. (2005) PMID:15713640 |
| Expression analysis of wild-type and transcription factor mutants in response to rapamycin | Transcript level changes in transcription factor mutants after rapamycin treatment, compared to the wild-type strain | transcription, cytoskeleton and molecular motors, chemical stimulus | 12 | Park D, et al. (2013) PMID:24349523 |
| Immunopurification results of cytoskeletal motor proteins in S. cerevisiae | The accompanying dataset is the result of a systematic study to identify the RNA cargoes associated with the cytoskeletal motor proteins of Saccharomyces cerevisiae | cytoskeleton and molecular motors | 118 | Casolari JM, et al. (2012) PMID:22359641 |
| Post-transcriptional regulation of stress response in a myo1_ mutant of the budding yeast Saccharomyces cerevisiae | The microarrays experiments of three biological and one technical replicates were performed in YJR12 (wt) and YJR13 (myo1__) strains | cytoskeleton and molecular motors, stress | 4 | Rivera-Ruiz ME, et al. (2010) PMID:21126371 |
| Pronounced and Extensive Microtubule Defects in a Saccharomyces cerevisiae DIS3 Mutant | The recently proposed exozyme hypothesis posits that subunits of the RNA processing exosome assemble into structurally distinct protein complexes that function in disparate cellular compartments and RNA metabolic pathways | cytoskeleton and molecular motors | 4 | Smith SB, et al. (2011) PMID:21919057 |