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CLA4-BEM1-CDC24 polarity complex Overview

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code.

Summary
Required for the establishment of cell polarity during the cell division cycle, and essential for bud emergence. Binds directly to activated CDC42 GTPase and is required for orchestrating a cellular gradient of CDC42. The complex assembles in late G1 and BEM1 directly augments the guanine exchange factor (GEF) activity of CDC24, promoting the exchange of CDC42-bound GDP with GTP. BEM1 also increases CDC24 phosphorylation by CLA4. Phosphorylation inhibits scaffold-dependent stimulation of CDC24 GEF activity, thus BEM1 stimulates GEF activity in a reversible fashion, controlling the flux of cellular signalling through CDC42. The core circuit appears to be a positive feedback loop with GTP-CDC42 at the membrane recruiting the complex from the cytoplasm to promote activation of neighbouring CDC42. A corresponding negative feedback loop appears to ensure only one bud forms.
GO Slim Terms

The yeast GO Slim terms are higher level terms that best represent the major S. cerevisiae biological processes, functions, and cellular components. The GO Slim terms listed here are the broader parent terms for the specific terms to which this gene product is annotated, and thus represent the more general processes, functions, and components in which it is involved.

enzyme regulator activity, guanyl-nucleotide exchange factor activity, kinase activity, transferase activity, biological process, site of polarized growth, incipient cellular bud site

Biological Process

Manually curated GO annotations reflect our best understanding of the basic biological process for this protein complex. Manually curated annotations are assigned by curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all manually curated GO annotations for accuracy and completeness.

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Qualifier Gene Ontology Term Annotation Extension Evidence Source Assigned On Reference

Molecular Function

Manually curated GO annotations reflect our best understanding of the basic molecular function for this protein complex. Manually curated annotations are assigned by curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all manually curated GO annotations for accuracy and completeness.

Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Qualifier Gene Ontology Term Annotation Extension Evidence Source Assigned On Reference

Cellular Component

Manually curated GO annotations reflect our best understanding of the basic cellular component for this protein complex. Manually curated annotations are assigned by curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all manually curated GO annotations for accuracy and completeness.

Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Qualifier Gene Ontology Term Annotation Extension Evidence Source Assigned On Reference

Shared Annotations

This diagram displays Gene Ontology terms (green) that are shared between the given macromolecular complex (black) and other yeast complexes (yellow). Note: No diagram is shown ("No shared annotations") if there are less than 2 shared GO annotations between this complex and any other complexes.