Gene Ontology Help

MUS81-MMS4 structure-specific endonuclease complex Overview

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code.

Structure-specific endonuclease that plays an important role in rescuing stalled replication forks and resolving the mitotic recombination intermediates. Has a substrate preference for branched DNA structures with a 5-prime end at the branch nick with cleavage probably occurring approximately half a helical turn upstream of the free 5-prime end. Resolves the four-way Holliday junction (HJ) in which the two recombining DNAs are covalently-linked junctions in crossover formation during meiotic recombination, however, its ability to cut intact HJs was found to be very limited in comparison with other structures, suggesting that a HJ precursor (such as a nicked HJ) might be its preferred DNA substrate. In mitotic cells, the activity of MUS81-MMS4 is low during S-phase, but phosphorylation of the MMS4 leads to complex activation at the onset of mitosis by the collaborative actions of two cell cycle kinases: CDC28 (P00546) and CDC5 (P32562).
GO Slim Terms

The yeast GO Slim terms are higher level terms that best represent the major S. cerevisiae biological processes, functions, and cellular components. The GO Slim terms listed here are the broader parent terms for the specific terms to which this gene product is annotated, and thus represent the more general processes, functions, and components in which it is involved.

hydrolase activity, nuclease activity, nuclear replication fork