Gene Ontology Help

ATG12-ATG5-ATG16 complex Overview

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code.

Essential for autophagy during nutrient deprivation, a catabolic process that sequesters undesired cellular material into autophagosomes for delivery to lysosomes for degradation. Functions as an E3-like enzyme in the ATG8 conjugation system. The ATG12-ATG5-ATG16 complex targets the autophagic membrane via the ATG5-ATG16 complex moiety and then recruits an ATG3:ATG8 thioester intermediate via the interaction between ATG3 and ATG12. The ATG12-ATG5 (CPX-1848) conjugate facilitates the transfer reaction of ATG8 from ATG3 to phosphatidylethanolamine (PE) through a reorganization of the catalytic centre of the E2-like enzyme ATG3. The C-terminal glycine of ATG8 is then conjugated to the amine moiety of PE. The role of ATG16, which has no E3-like activity appears to be to target the ATG12-ATG5 conjugate to the autophagic membranes. ATG8-PE/ATG12-ATG5 complexes form homogeneous oligomers, comprising two to four subunits. ATG16 may then act to reorganize ATG8-PE/ATG12-ATG5 oligomers to form a continuous, flat protein layer with meshwork-like architecture on membranes.
GO Slim Terms

The yeast GO Slim terms are higher level terms that best represent the major S. cerevisiae biological processes, functions, and cellular components. The GO Slim terms listed here are the broader parent terms for the specific terms to which this gene product is annotated, and thus represent the more general processes, functions, and components in which it is involved.

catabolic process, cellular component assembly, organelle assembly, protein lipidation, vacuole organization, membrane, Atg12-Atg5-Atg16 complex