Gene Ontology Help

RNA polymerase I upstream activating factor complex Overview

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code.

Binds tightly to the upstream element of the yeast Pol I promoter and is essential for a high level of transcription of the 35S rRNA gene. UAF has dual functions: both as a positive transcription factor for Pol I, and as a silencer for Pol II transcription of rRNA genes. Deletion of any one of the subunit protein genes encoding essential nonhistone proteins causes derepression of Pol II transcription of the 35S rRNA genes. The association of H3 and H4 with the other UAF components is important for the stability of UAF in vivo and, hence, for rRNA transcription by Pol I. Initiation of transcription requires the assembly of UAF, the core factor (CPX-1836), the TATA binding protein SPT15 (P13393), and RNAP-I (CPX-1664) with RRN3 (P36070) on the upstream element and core promoter. Upon transcription initiation, UAF, RRN3 and CF dissociate from the promoter.
GO Slim Terms

The yeast GO Slim terms are higher level terms that best represent the major S. cerevisiae biological processes, functions, and cellular components. The GO Slim terms listed here are the broader parent terms for the specific terms to which this gene product is annotated, and thus represent the more general processes, functions, and components in which it is involved.

DNA binding, DNA-templated transcription, biosynthetic process, cellular nitrogen compound metabolic process, transcription by RNA polymerase I, nucleus, organelle, RNA polymerase I transcription regulator complex, RNA polymerase I upstream activating factor complex, transcription regulator complex