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Anaphase-Promoting Complex, CDC20 variant Overview

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code.


Summary
APC, a key regulator of cell cycle progression, is a conserved cullin-RING E3 ubiqutin ligase which facilitates multiubiqutination of cell cycle proteins, including cyclins, so marking them for proteasomal destruction. Generally believed that APC acts as a scaffold that brings E2 enzymes and substrates into close proximity. APC activity is required for metaphase-anaphase transition, mitotic exit, G1 phase and DNA replication. Targets securin for destruction, which is necessary for chromosome segregation. APC seems to have similar function in meiosis. Co-activators, CDC20 and CDH1, associate with APC core complex (CPX-756) at specific stages of cell cycle, and are thought to be involved in substrate specificity. APC-Cdc20 is active in presence of high cyclin-cdk activity in M phase but after metaphase when cyclin-cdk activity decreases, CDH1 is dephosphorylated, CDC20 is degraded and APC-Cdh1 (CPX-761) activated. Ama1 (CPX-762) is meiotic co-activator required for sporulation and contributes to securin degradation and cyclin Clb5 in anaphase of meiosis. All APC co-activators, characterized by the presence of sequence elements, C-box and the IR-tail, that mediate their binding to APC, contain a C-terminal WD40 domain, predicted to fold into a propeller-like structure, believed to recognize APC substrates by interacting with specific recognition elements in substrates, D-boxes and KEN-boxes. Genetic inactivation of APC is lethal.
GO Slim Terms

The yeast GO Slim terms are higher level terms that best represent the major S. cerevisiae biological processes, functions, and cellular components. The GO Slim terms listed here are the broader parent terms for the specific terms to which this gene product is annotated, and thus represent the more general processes, functions, and components in which it is involved.

transferase activity, catabolic process, protein modification by small protein conjugation or removal, proteolysis involved in protein catabolic process, regulation of cell cycle, anaphase-promoting complex, ubiquitin ligase complex