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Complex: Nuclear/nucleolar exosome complex, DIS3-RRP6 variant

Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.

Curated by
IntAct
ComplexAc
CPX-599
Systematic Name
CSL4:DIS3:MTR3:RRP4:RRP6:RRP40:RRP42:RRP43:RRP45:RRP46:SKI6
SGD ID
SGD:S000217582
Description
3' to 5' exo- and endoribonuclease complex that is involved in RNA maturation and degradation which include the removal of unstable and aberrant transcripts and may also regulate the levels of specific transcripts in response to environmental factors. Restricted to processing linear and circular single-stranded RNAs only. RNAs with complex secondary structures, particularly at the 3' end of the molecule, may have to be unwound or pre-processed by co-factors prior to entering the complex. Although some RNAs may be targeted directly to the catalytic subunits, the majority of substrates enter the barrel-like structure of the exosome through a pore at the centre of the cap and are threaded through the central channel in a 3' to 5' orientation. The ribonuclease activity of the catalytic subunits facilitates the degradation process. Two different exosomes exist in yeast, one found in the nucleus and nucleolus (this complex), the other form is lacking the RRP6 subunit and is found in the cytosol (CPX-603). The nuclear/nucleolar RNA exosome is involved in a) proper maturation of most RNA species such as intron-removal from pre-mRNAs and tRNA precursors and rRNA, snRNA, snoRNA, lncRNA and enhancer RNA processing, especially the removal of their 3-prime ends, b) the elimination of RNA processing by-products and non-coding, cryptic transcripts, such as upstream antisense RNA species (uaRNA), enhancer RNAs (eRNAs), heterochromatin-forming repetitive elements (ribosomal DNA repeats and centromeres) and long non-coding RNAs, c) the elimination of mRNAs with processing defects and mRNAs that fail to undergo proper splicing or 3′ end formation and d) gene expression either by mRNA processing or coordination of intron retention leading to regulation of decay of otherwise intact mRNAs. Nuclear exosome activity therefore limits or excludes export of target RNAs to the cytoplasm. Possibly also involved in the degradation of mRNAs with defects in their co-transcriptional packaging into ribonucleoprotein particles (mRNPs), retention of aberrant transcripts on the chromatin and transcription termination or DNA damage repair processes.

Complex Diagram

This diagram displays the protein subunits (blue) of the complex and how they interact with each other. Interactions with other relevant participants such as small molecules (purple), sub-complexes (yellow), and other subunits (red) are also shown. If a participant appears in the diagram with no lines connected to it, then its specific interactions with other participants are currently unknown.

PDB Structure

When available from the Protein Data Bank (PDB), the physical structure of the macromolecular complex is displayed. Click on the image or link for further information about the physical structure.

2WP8 2WP8 4IFD 4IFD 4OO1 4OO1 5C0X 5C0X

Subunits

This table lists all participants of the complex (proteins, small molecules, nucleic acids, etc.) and their respective stoichiometry. A brief description is also provided for each participant in the table.

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Subunit Description Stoichiometry

Gene Ontology

Gene Ontology (GO) terms that describe the function of a complex, the biological process in which it participates, or its cellular location. Click on a term for its definition and other objects annotated to that term.

Shared Annotations

This diagram displays Gene Ontology terms (green) and subunits (blue) that are shared between the given macromolecular complex (black) and other yeast complexes (yellow). The shared GO terms or subunits can be visualized separately using the radio button at the bottom of the graph. Note: No diagram is shown ("No shared annotations") if there are less than 2 shared annotations (either GO terms or subunits of other complexes) between this complex and any other complexes.

References

List of references used specifically to curate the information on this page.

External Identifiers

IntEnz: 3.1.13 | IntEnz: 3.1.26

Resources

IntAct | ModelArchive | PDB:2WP8 | PDB:4IFD | PDB:4OO1 | PDB:5C0X