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Cleavage and polyadenylation specificity factor complex Overview

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code.


Summary
Endonuclease complex required for mRNA 3' end processing to form a defined 3' end of the transcribed RNA. The complex cleaves pre-mRNAs, adds a polyadenylate tail, and triggers transcription termination. The 3' end of mature mRNAs is generated by a site-specific endonucleolytic cleavage of an internal phosphodiester bond of the primary transcript by YSH1. The upstream cleavage product generated is then polyadenylated by PAP1 to form a 50-90 adenosine tail at its 3-prime hydroxyl end, which is required for nuclear export, translation, and stability of mRNA. The downstream cleavage product is rapidly degraded. Cleavage and polyadenylation cycles are regulated by phosphorylation/dephosphorylation. Phosphorylation of CPF is inhibitory to polyadenylation therefore dephosphorylation of CPF by GLC7 is required to switch the processing complex to one competent for poly(A) addition.
GO Slim Terms

The yeast GO Slim terms are higher level terms that best represent the major S. cerevisiae biological processes, functions, and cellular components. The GO Slim terms listed here are the broader parent terms for the specific terms to which this gene product is annotated, and thus represent the more general processes, functions, and components in which it is involved.

RNA binding, hydrolase activity, ion binding, nuclease activity, nucleotidyltransferase activity, phosphatase activity, transferase activity, DNA-templated transcription termination, biosynthetic process, mRNA processing, nucleus, organelle