A transcription terminator for RNA polymerase I (polI) in the yeast, Saccharomyces cerevisiae, is composed of two essential elements, the 11bp binding site for Reb1p and an upstream T-rich element coding for the last 10-12 nucleotides of the terminated transcript. We now show that, if the upstream element is changed to homopolymer T residues, polI undergoes iterative slippage, long poly(U) tails are added to the transcript, and termination is impaired. Reinsertion of one or two non-T residues within a critical region prevents iterative slippage and reinstates termination. A survey of naturally occurring terminators reveals that many contain T-rich upstream regions with non-T residues situated appropriately to prevent slippage. We discuss the possibility that the first step in slippage, backward sliding of both the transcript and the catalytic center of the polymerase, may be an obligatory step in the normal termination process.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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