RAD52 / YML032C Overview

Standard Name
RAD52 1
Systematic Name
Feature Type
ORF , Verified
Protein involved in homologous recombination; attenuates resection of DNA double-strand break ends and stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA (ssDNA); anneals complementary ssDNA; involved in the repair of double-strand breaks in DNA during vegetative growth, meiosis and UV induced sister chromatid recombination; forms a homodecameric ring; phosphorylation by Cdc28p in G2/M phase promotes Rad52p ring interaction that leads to superstructure formation 2 3 4 5 6 7 8 9
Name Description
RADiation sensitive 1
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

RAD52 is located on the left arm of chromosome XIII between SRC1 inner nuclear membrane protein and NDC1 nuclear pore subunit; coding sequence is 1416 nucleotides long with 14 SNPs, 4 of which cause amino acid polymorphisms
Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Rad52p is 471 amino acids long, shorter-lived, low in abundance; phosphorylated on 13 residues, sumoylated on 13 residues
Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)
2164 +/- 343
Half-life (hr)


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.

View all RAD52 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Recombinase involved in the repair of DNA double-strand breaks and single-strand postreplication gaps, as well as the formation of meiotic four-stranded branched intermediates

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated
Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Non-essential gene; null mutant is sensitive to DNA damaging agents, DNA synthesis and topoisomerase inhibitors; null mutant is sensitive to X-rays, gamma rays and UV radiation; null displays increased chromosome instability including gross chromosomal rearrangements, increased mutation rate, elevated Ty element transposition and decreased mitotic and ectopic recombination in meiosis; null mutant is temperature sensitive, and has decreased growth rate and competitive fitness on rich and synthetic media; null mutant has decreased replicative lifespan, increased desiccation sensitivity and decreased resistance to zinc deficiency; null contains large-budded cells with nuclear extensions at the nuclear envelope, adjacent to the nucleolus; homozygous reduction of function mutant has a sporulation defect, and the heterozygous null mutant is haploinsufficient
Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

Rad52p interacts physically with proteins involved in DNA repair; RAD52 interacts genetically with genes involved in DNA repair

1601 total interactions for 593 unique genes

Physical Interactions

  • Affinity Capture-MS: 36
  • Affinity Capture-RNA: 8
  • Affinity Capture-Western: 40
  • Biochemical Activity: 6
  • Co-crystal Structure: 2
  • Co-localization: 8
  • Co-purification: 1
  • FRET: 1
  • PCA: 1
  • Reconstituted Complex: 28
  • Two-hybrid: 35

Genetic Interactions

  • Dosage Growth Defect: 2
  • Dosage Lethality: 4
  • Dosage Rescue: 19
  • Negative Genetic: 472
  • Phenotypic Enhancement: 161
  • Phenotypic Suppression: 118
  • Positive Genetic: 116
  • Synthetic Growth Defect: 331
  • Synthetic Lethality: 128
  • Synthetic Rescue: 84
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

RAD52 transcription is upregulated by Rpn4p during DNA damage response; Rad52 protein activity is upregulated by Cdc28p during G2/M transition of mitotic cell cycle
Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.

Last Updated: 2003-01-06

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.