PIL1 / YGR086C Overview


Standard Name
PIL1 1 2
Systematic Name
YGR086C
SGD ID
SGD:S000003318
Feature Type
ORF , Verified
Description
Eisosome core component involved in endocytosis; mitochondria phosphoprotein that localizes to the outer membrane and is involved in maintaining mitochondrial morphology and mitophagy; phosphorylated (T233) upon Pkc1p hyperactivation in a Slt2p MAPK-dependent fashion; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; BAR domain family member; protein increases in abundance and relocalizes from the plasma membrane to cytoplasm upon DNA replication stress 1 3 4 5 6 7 8
Name Description
Phosphorylation Inhibited by Long chain bases 1
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Mitochondrial phosphoprotein; phosphorylated (T233) upon Pkc1p hyperactivation in a Slt2p MAPK-dependent fashion; BAR domain family member; protein increases in abundance and relocalizes from the plasma membrane to cytoplasm upon DNA replication stress
Length (a.a.)
339
Mol. Weight (Da)
38319.3
Isoelectric Point
4.26
Median Abundance (molecules/cell)
29832 +/- 9050
Half-life (hr)
6.4

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all PIL1 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Lipid-binding protein involved in protein localization, endocytosis and eisosome assembly; also involved in negative regulation of protein kinase activity and the response to heat; localizes to the eisosome

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null mutant has an abnormal plasma membrane, with defective eisosome organization and aberrant localization of eisosome proteins; null mutant is sensitive to rapamycin and H2O2, has reduced endocytosis, and has increased innate thermotolerance; in large-scale studies, null mutant has reduced competitive fitness in a variety of different media, is sensitive to osmotic stress, and has altered resistance to a variety of chemicals
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


810 total interactions for 611 unique genes

Physical Interactions

  • Affinity Capture-MS: 533
  • Affinity Capture-RNA: 9
  • Affinity Capture-Western: 9
  • Biochemical Activity: 2
  • Co-crystal Structure: 1
  • Co-localization: 1
  • Co-purification: 1
  • Cross-Linking-MS (XL-MS): 1
  • FRET: 3
  • PCA: 7
  • Proximity Label-MS: 3
  • Reconstituted Complex: 1
  • Two-hybrid: 9

Genetic Interactions

  • Dosage Growth Defect: 3
  • Negative Genetic: 176
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 3
  • Positive Genetic: 31
  • Synthetic Growth Defect: 6
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 1
  • Synthetic Rescue: 3
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Regulators
13
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
52
Additional
69
Reviews
23

Resources