SNF1 / YDR477W Overview

Standard Name
SNF1 1
Systematic Name
CAT1 56 , CCR1 58 , GLC2 66 , HAF3 67 , PAS14 68
Feature Type
ORF , Verified
AMP-activated S/T protein kinase (AMPK); complexes with Snf4p and a Sip1p/Sip2p/Gal83p family member; required for glucose-shortage induced respiratory gene expression, heat shock, sporulation, and peroxisome biogenesis; regulates filamentous growth; acts as a non-canonical GEF activating Arf3p during invasive growth; role in mitotic spindle alignment in non-limiting glucose; phosphorylates Kcs1p; target of SUMO-dependent, Ub-mediated destruction; polyhistidine tract acts as a pH sensing module 2 3 5 6 7 8 9 10 11
Name Description
Sucrose NonFermenting 4
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

SNF1 is located on the right arm of chromosome IV near the telomere between uncharacterized gene YDR476C and SNM1 ribonuclease MRP subunit; coding sequence is 1902 nucleotides long with 12 SNPs, one of which causes a His/Asn polymorphism at residue 28
Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Snf1p is 633 amino acids long, of moderate half-life, and low-to-moderate abundance; acetylated on K59 and K68, ubiquitinylated on 6 lysines, phosphorylated on 31 residues
Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)
5622 +/- 2394
Half-life (hr)


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.

View all SNF1 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

AMP-activated serine/threonine kinase subunit of the AMP-activated protein kinase complex; also serves as guanine nucleotide exchange factor (GEF) specific for Arf3p in response to glucose depletion; regulates both pseudohyphal and filamentous growth in response to starvation and carbohydrate metabolic process; inhibits translation of GCN4; involved in mitotic spindle alignment along the mother-bud axis

View computational annotations

Cellular Component

Manually Curated


Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.

Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Non-essential gene; mutants are defective in glucose-dependent regulation of transcription, with pleiotropic effects on carbon source utilization, respiratory growth, cell wall structure, thermotolerance, hyperosmotic stress and starvation sensitivity, autophagy, peroxisome biogenesis, filamentous growth, sporulation in diploids

Classical Genetics

reduction of function
gain of function

Large-scale Survey

reduction of function
Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

Snf1p interacts physically with proteins involved in transcription; SNF1 interacts genetically with genes involved in transcription

1425 total interactions for 848 unique genes

Physical Interactions

  • Affinity Capture-MS: 137
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 65
  • Biochemical Activity: 127
  • Co-crystal Structure: 2
  • Co-fractionation: 1
  • Co-localization: 6
  • Co-purification: 2
  • PCA: 5
  • Reconstituted Complex: 41
  • Two-hybrid: 92

Genetic Interactions

  • Dosage Growth Defect: 8
  • Dosage Lethality: 1
  • Dosage Rescue: 36
  • Negative Genetic: 627
  • Phenotypic Enhancement: 26
  • Phenotypic Suppression: 44
  • Positive Genetic: 77
  • Synthetic Growth Defect: 46
  • Synthetic Lethality: 9
  • Synthetic Rescue: 69
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

SNF1 promoter is bound by Reb1p in response to heat; SNF1 transcription is regulated by Fkh1p, Gcn4p, and Sin4p; Snf1p activity is regulated by Ppz1p and upregulated by Sak1p
Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.

Last Updated: 2006-03-27

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.