LYS2 / YBR115C Overview


Standard Name
LYS2 1
Systematic Name
YBR115C
SGD ID
SGD:S000000319
Feature Type
ORF , Verified
Description
Alpha aminoadipate reductase; catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p 2
Name Description
LYSine requiring 3
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
LYS2 is located on the right arm of chromosome II between RAD16 nucleotide excision repair protein and TKL2 transketolase; coding sequence is 4179 nucleotides long with 30 SNPs, 9 of which cause amino acid polymorphisms
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Lys2p is 1392 amino acids long, low-to-moderate in abundance; contains an AMP-binding domain, a phosphopantetheine-binding domain, and an NAD-binding domain; ubiquitinylated on 3 lysines, phosphorylated on 3 residues
Length (a.a.)
1392
Mol. Weight (Da)
155321.8
Isoelectric Point
5.75
Median Abundance (molecules/cell)
9748 +/- 4016

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all LYS2 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Cytoplasmic L-aminoadipate-semialdehyde dehydrogenase involved in lysine biosynthesis

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated

Pathways


Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null mutant requires lysine for growth and is sensitive to acetic acid; lys2-402 mutant is able to utilize alpha-aminoadipate as nitrogen source; in large-scale studies null mutant displays altered resistance to various chemicals
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
Lys2p interacts physically with proteins involved in chromatin organization; LYS2 interacts genetically with genes involved in RNA catabolism and chromatin organization

38 total interactions for 33 unique genes

Physical Interactions

  • Affinity Capture-MS: 14
  • Affinity Capture-RNA: 3
  • Co-localization: 1
  • Co-purification: 1
  • Proximity Label-MS: 1

Genetic Interactions

  • Dosage Growth Defect: 1
  • Positive Genetic: 1
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 2
  • Synthetic Rescue: 11
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
LYS2 promoter is bound by Fkh2p and Stp1p in response to heat; LYS2 transcription is regulated by Sfp1p in response to stress; LYS2 transcription is upregulated by Gcn4p in response to boron
Regulators
4
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.


Last Updated: 2007-10-04

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
34
Additional
163
Reviews
10

Resources