NRG2 / YBR066C Overview

Standard Name
NRG2 1
Systematic Name
Feature Type
ORF , Verified
Transcriptional repressor; mediates glucose repression and negatively regulates filamentous growth; activated in stochastic pulses of nuclear localization in response to low glucose 2 3 4
Name Description
Negative Regulator of Glucose-controlled genes 1
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)
1086 +/- 382


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.

View all NRG2 alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Sequence-specific DNA binding protein involved in negative regulation of pseudohyphal growth and transcription by RNA polymerase II; localizes to nucleus

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated
Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

The nrg2 null mutant is viable; the null mutant of paralog nrg1 is viable; the nrg2 nrg1 double mutant displays a phenotypic enhancement.

76 total interactions for 63 unique genes

Physical Interactions

  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 5
  • Affinity Capture-Western: 1
  • Two-hybrid: 1

Genetic Interactions

  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Negative Genetic: 49
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 1
  • Positive Genetic: 5
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 1
  • Synthetic Rescue: 4
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

NRG2 encodes a transcriptional repressor that is a member of the C2H2 zinc finger class. Nrg2p, along with its paralog Nrg1p, responds to glucose signals received via Snf1p kinase, and to stress signals received via the Rim101p transcriptional repressor. Nrg1p and Nrg2p regulate a variety of processes, including response to glucose levels; response to alkaline pH and to salt, osmotic, and oxidative stress; filamentous growth; and biofilm formation. Some major targets of Nrg1p and Nrg2p include FLO11, encoding a cell surface adhesin, ENA1, encoding a plasma membrane salt efflux pump, and SUC2, encoding extracellular invertase. The set of genes regulated by Nrg2p overlaps with, but is not identical to, the set regulated by Nrg1p. Nrg2p represses transcription by recruiting the Cyc8p-Tup1p complex to promoters. NRG2 transcription does not respond to glucose levels and is up-regulated at alkaline pH, in contrast to NRG1 transcription which is glucose-repressed and up-regulated at acidic pH. Stability of the NRG2 mRNA is negatively regulated via mRNA decay.
Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.