ATG8 / YBL078C Overview
- Standard Name
- ATG8 1
- Systematic Name
- YBL078C
- SGD ID
- SGD:S000000174
- Aliases
- APG8 2 27 , AUT7 14 , CVT5 39
- Feature Type
- ORF , Verified
- Description
- Ubiquitin-like protein conjugated to phosphatidylethanolamine (PE); role in membrane fusion and phagophore expansion during autophagosome formation; deconjugation is also required for biogenesis; targets Atg1p to autophagosomes; binds to the Atg1p-Atg13p complex, triggers its vacuolar degradation; role in vacuolar membrane protein turnover during early stationary phase; component of autophagosomes and Cvt vesicles; targeted to vacuole via AP-3 pathway 2 3 4 5 6 7 8 9 10 11 12
- Name Description
- AuTophaGy related 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Atg8p is 117 amino acids long, short-lived, low in abundance; acetylated on M1, sumoylated on K6 and K15
- Length (a.a.)
- 117
- Mol. Weight (Da)
- 13630.9
- Isoelectric Point
- 9.59
- Median Abundance (molecules/cell)
- 2400 +/- 1536
- Half-life (hr)
- 5.4
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all ATG8 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Protein tag or marker; involved in macroautophagy, cytoplasm-to-vacuole targeting (CVT) pathway, late nucleophagy, piecemeal microautophagy of nucleus, autophagic vacuole assembly, ER to Golgi vesicle-mediated transport and mitochondrion degradation; localizes to pre-autophagosomal structure (PAS), vacuolar membrane
View computational annotations
Molecular Function
- Manually Curated
- enables phosphatidylethanolamine binding (IMP)
- enables protein tag activity (IMP)
Biological Process
- Manually Curated
- involved in autophagy of mitochondrion (IMP)
- involved in cytoplasm to vacuole targeting by the Cvt pathway (IMP)
- involved in endoplasmic reticulum to Golgi vesicle-mediated transport (IPI, IGI, IMP)
- involved in lipid droplet formation (IMP)
- involved in membrane fusion (IDA, IMP)
- involved in nucleophagy (IMP)
- involved in piecemeal microautophagy of the nucleus (IMP)
- involved in protein localization to phagophore assembly site (IMP)
- involved in protein targeting to vacuole involved in autophagy (IMP)
- involved in protein-containing complex localization (IMP)
- involved in regulation of macroautophagy (IMP)
- involved in regulation of membrane invagination (IMP)
- involved in reticulophagy (IMP)
Cellular Component
- Manually Curated
- located in autophagosome (IDA)
- located in cytosol (HDA)
- located in fungal-type vacuole membrane (IDA, IPI)
- is active in lipid droplet (IDA)
- located in phagophore assembly site (IDA)
- located in vacuole-isolation membrane contact site (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant shows no autophagy, mitophagy or pexophagy, decreased chronological and replicative lifespan, increased sensitivity to nitrogen starvation and is unable to sporulate; also shows defects in vacuolar transport and decreased rate of respiratory growth
Classical Genetics
- reduction of function
- null
- autophagy: absent
- autophagy: decreased
- cell death: absent
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chronological lifespan: decreased
- fermentative metabolism: decreased
- growth in exponential phase: decreased rate
- inviable
- late nucleophagy: absent
- metal resistance: increased
- mitochondrial rho- mutation frequency: increased
- mitophagy: absent
- mitophagy: decreased
- nuclear morphology: abnormal
- pexophagy: absent
- protein secretion: absent
- protein/peptide accumulation: increased
- protein/peptide distribution: abnormal
- protein/peptide modification: abnormal
- protein/peptide modification: absent
- replicative lifespan: decreased
- replicative lifespan: increased
- resistance to chemicals: decreased
- respiratory growth: decreased rate
- reticulophagy: decreased
- sporulation: absent
- starvation resistance: decreased
- subcellular morphology: abnormal
- toxin resistance: decreased
- vacuolar morphology: abnormal
- vacuolar transport: absent
- vegetative growth: decreased
- viability: decreased
- repressible
- null
- chemical compound accumulation: increased
- chronological lifespan: decreased
- competitive fitness: decreased
- haploinsufficient
- oxidative stress resistance: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- reticulophagy: decreased
- sporulation efficiency: decreased
- sporulation: absent
- starvation resistance: decreased
- stress resistance: decreased
- utilization of nitrogen source: decreased rate
- vacuolar morphology: abnormal
- viable
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Atg8p interacts physically with proteins involved in Golgi vesicle transport; ATG8 interacts genetically with genes involved in protein targeting
1110 total interactions for 778 unique genes
Physical Interactions
- Affinity Capture-MS: 136
- Affinity Capture-RNA: 4
- Affinity Capture-Western: 72
- Biochemical Activity: 14
- Co-crystal Structure: 8
- Co-fractionation: 1
- Co-localization: 10
- Co-purification: 2
- PCA: 7
- Protein-peptide: 2
- Proximity Label-MS: 514
- Reconstituted Complex: 38
- Two-hybrid: 28
Genetic Interactions
- Dosage Lethality: 2
- Dosage Rescue: 3
- Negative Genetic: 218
- Phenotypic Enhancement: 13
- Phenotypic Suppression: 12
- Positive Genetic: 17
- Synthetic Growth Defect: 9
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 20
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2008-04-25
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.