HSP82 / YPL240C Overview

Standard Name
HSP82 1 2
Systematic Name
HSP90 34
Feature Type
ORF , Verified
Hsp90 chaperone; functionally redundant with Hsc82p; required for pheromone signaling; negative regulator of the Hsf1p-dependent heat shock response with Cpr7p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; promotes solubility of chaperone-substrate complexes; DNA damage induced nuclear import is Aha1p dependent; protein abundance increases in response to DNA replication stress; human homolog, HSP90AB1, complements null mutant 3 4 5 6 7 8 9 11 12 13 14
Name Description
Heat Shock Protein 10
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

HSP82 is on the left arm of chromosome XVI between CIN2 GTPase-activating protein and YAR1 nucleocytoplasmic shuttling assembly chaperone; coding sequence is 2130 nucleotides long with many, many SNPs; HSP82 has paralog HSC82 that arose from the whole genome duplication
Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Hsp82p is 709 amino acids long, very long-lived, and high in abundance; contains 7 Hsp90 domains; undergoes various post-translational modifications including methylation, acetylation, phosphorylation, succinylation, sumoylation, and ubiquitinylation at 29 residues; protein abundance increases in response to DNA replication stress
Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)
86723 +/- 51088
Half-life (hr)


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.

View all HSP82 alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Unfolded protein-binding ATPase (chaperone); involved in folding of nascent proteins, mitochondrial targeting, and assembly of multiprotein complexes, such as proteasome and box C/D snoRNP; localizes in cytoplasm

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated


Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.

Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Non-essential gene; null mutant is sensitive to freeze-thaw; some point mutations cause sensitivity to heat, hyperosmotic stress, Calcofluor White, geldanamycin and radicicol, decreased competitive fitness, slow exponential growth and decreased budding
Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

The hsp82 null mutant is viable; the null mutant of paralog hsc82 is viable; the hsp82 hsc82 double mutant is inviable or displays a growth defect; Hsp82p interacts physically with proteins involved in translation, HSP82 interacts genetically with genes involved in transcription

2981 total interactions for 1864 unique genes

Physical Interactions

  • Affinity Capture-MS: 1202
  • Affinity Capture-RNA: 13
  • Affinity Capture-Western: 115
  • Biochemical Activity: 8
  • Co-crystal Structure: 7
  • Co-localization: 1
  • Co-purification: 3
  • FRET: 2
  • PCA: 1
  • Protein-peptide: 2
  • Proximity Label-MS: 3
  • Reconstituted Complex: 76
  • Two-hybrid: 226

Genetic Interactions

  • Dosage Growth Defect: 1
  • Dosage Lethality: 2
  • Dosage Rescue: 14
  • Negative Genetic: 20
  • Phenotypic Enhancement: 5
  • Phenotypic Suppression: 15
  • Positive Genetic: 2
  • Synthetic Growth Defect: 968
  • Synthetic Lethality: 279
  • Synthetic Rescue: 16
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

Transcription upregulated by Hsf1p in response to heat; protein activity regulated by Ppt1p in response to starvation
Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.

Last Updated: 2006-06-12

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.