CUE1 / YMR264W Overview


Standard Name
CUE1 1
Systematic Name
YMR264W
SGD ID
SGD:S000004877
Aliases
KIS4 5
Feature Type
ORF , Verified
Description
Ubiquitin-binding protein; ER membrane protein that recruits and integrates the ubiquitin-conjugating enzyme Ubc7p into ER membrane-bound ubiquitin ligase complexes that function in the ER-associated degradation (ERAD) pathway for misfolded proteins; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination and to promote diubiquitin elongation, facilitating polyubiquitin chain formation 1 2 3 4
Name Description
Coupling of Ubiquitin conjugation to ER degradation 1
Paralog
CUE4
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
203
Mol. Weight (Da)
22760.9
Isoelectric Point
6.69
Median Abundance (molecules/cell)
3719 +/- 2487
Half-life (hr)
8.5

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all CUE1 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Ubiquitin-protein transferase activator involved in ubiquitin-dependent protein catabolic processes associated with the endoplasmic reticulum (ER) and localization of proteins to the ER membrane; an integral component of the ER membrane and associated with both Doa10p and Hrd1p ubiquitin ligase complexes

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated

Complex

Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.


Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null mutation causes elevated accumulation of reporter proteins and confers increased sensitivity to nickel ions; in systematic studies mutants show increased sensitivity to camptothecin MMS, amitrole and Congo Red
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
The cue1 null mutant is viable; the null mutant of paralog cue4 is viable; the cue1 cue4 double mutant has not been annotated for phenotype.

307 total interactions for 191 unique genes

Physical Interactions

  • Affinity Capture-MS: 23
  • Affinity Capture-RNA: 6
  • Affinity Capture-Western: 9
  • Co-crystal Structure: 1
  • Co-fractionation: 1
  • Co-purification: 3
  • PCA: 14
  • Reconstituted Complex: 9

Genetic Interactions

  • Dosage Lethality: 1
  • Dosage Rescue: 5
  • Negative Genetic: 119
  • Phenotypic Enhancement: 19
  • Phenotypic Suppression: 27
  • Positive Genetic: 45
  • Synthetic Growth Defect: 13
  • Synthetic Lethality: 3
  • Synthetic Rescue: 9
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Regulators
4
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
32
Additional
36
Reviews
26

Resources