MOT3 / YMR070W Overview


Standard Name
MOT3 1
Systematic Name
YMR070W
SGD ID
SGD:S000004674
Aliases
ROX7 , [MOT3+] 12 , [MOT3]
Feature Type
ORF , Verified
Description
Transcriptional repressor, activator; role in cellular adjustment to osmotic stress including modulation of mating efficiency; involved in repression of subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of Tup1p-Cyc8p general repressor to promoters; relocalizes to cytosol under hypoxia; forms [MOT3+] prion under anaerobic conditions 2 3 4 5 6 7 8 9 10 11
Name Description
Modifier of Transcription 1
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
490
Mol. Weight (Da)
54387.6
Isoelectric Point
8.98
Median Abundance (molecules/cell)
2570 +/- 845

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all MOT3 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Sequence-specific DNA-binding RNA polymerase II transcription factor involved in the cellular response to osmotic stress; positively regulates transcription in response to osmotic stress and negatively regulates transcription of ergosterol biosynthetic genes and the hypoxic gene ANB1; localizes to the nucleus and cytosol

View computational annotations

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


454 total interactions for 334 unique genes

Physical Interactions

  • Affinity Capture-MS: 30
  • Affinity Capture-RNA: 7
  • Affinity Capture-Western: 2
  • PCA: 2
  • Protein-RNA: 1
  • Reconstituted Complex: 5
  • Two-hybrid: 7

Genetic Interactions

  • Dosage Rescue: 6
  • Negative Genetic: 324
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 1
  • Positive Genetic: 45
  • Synthetic Growth Defect: 8
  • Synthetic Lethality: 6
  • Synthetic Rescue: 7
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
MOT3 encodes a zinc finger transcription factor of the beta-beta-alpha family, and binds 5'-HAGGYA-3' motifs. Mot3p is a globally acting, environmentally responsive regulator that modulates a variety of processes, including mating, carbon metabolism, stress response, maintenance of cell wall composition, and pheromone signaling. Mot3p forms a co-repressor complex with Rox1p that affects many cellular processes, including the repression of genes involved in ergosterol biosynthesis and of anaerobic genes, such as DAN1, during aerobic growth. Mot3p regulates cell-wall remodeling during adaptation to anaerobic conditions. Mot3p can form a prion called [MOT3+], which naturally appears at a detectable frequency. [MOT3+] cannot carry out normal functions of Mot3p, and is likely to have far-reaching phenotypic effects, which may be advantageous under certain environmental conditions.
Regulators
12
Targets
59
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
44
Additional
57
Reviews
53

Resources