CDC6 / YJL194W Overview

Standard Name
CDC6 1
Systematic Name
Feature Type
ORF , Verified
Essential ATP-binding protein required for DNA replication; component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; dual role as a mitotic cyclin-dependent kinase inhibitor, that binds to and inhibits Cdc28p/Clb2p along with Sic1p, contributing to mitotic exit; homologous to S. pombe Cdc18p; relocalizes from nucleus to cytoplasm upon DNA replication stress; degraded in response to plasma membrane stress 2 4 5 6 7 8
Name Description
Cell Division Cycle 3
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

CDC6 is located on the left arm of chromosome X between ELO1 elongase and uncharacterized gene YJL193W; dubious ORF overlaps the 5' half of CDC on the opposite strand; coding sequence is 1542 nucleotides long with 15 SNPs, 2 of which cause amino acid polymorphisms
Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Cdc6p is 513 amino acids long, low in abundance; component of the pre-replicative complex (pre-RC); relocalizes from nucleus to cytoplasm upon DNA replication stress; degraded in response to plasma membrane stress; phosphorylated on 8 residues
Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)
2353 +/- 1123


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.

View all CDC6 alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

ATPase and GTPase; binds chromatin and DNA replication origins; involved in G1/S transition of the mitotic cell cycle and pre-replicative complex assembly during DNA replication; regulates DNA replication initiation and chromatin silencing at telomeres; subunit of the nuclear pre-replicative complex and the DNA replication preinitiation complex

View computational annotations

Cellular Component

Manually Curated
Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Essential gene; repressible mutant arrests in S phase with partially replicated DNA; conditional mutant arrests with large budded cells and nuclei situated at the mother-bud neck; conditional mutant displays chromosomal instability with an elevated rate of chromosome loss and gross chromosomal rearrangements at semi-permissive temperatures; conditional mutant displays an increased mutation frequency at permissive temperature; overexpression results in a decreased rate of vegetative growth
Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

Cdc6p interacts physically with proteins involved in DNA replication; CDC6 interacts genetically with genes involved in DNA repair

553 total interactions for 328 unique genes

Physical Interactions

  • Affinity Capture-MS: 16
  • Affinity Capture-RNA: 7
  • Affinity Capture-Western: 25
  • Biochemical Activity: 16
  • Co-localization: 6
  • Co-purification: 4
  • Protein-peptide: 1
  • Protein-RNA: 1
  • Reconstituted Complex: 33
  • Two-hybrid: 23

Genetic Interactions

  • Dosage Growth Defect: 3
  • Dosage Lethality: 11
  • Dosage Rescue: 11
  • Negative Genetic: 252
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 6
  • Positive Genetic: 41
  • Synthetic Growth Defect: 43
  • Synthetic Lethality: 31
  • Synthetic Rescue: 17
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

CDC6 promoter is bound by Hfi1p, Put3p, and Swi4p in response to heat; Cdc6 protein stability is increased by CLB2-CDC28 kinase complex and decreased by serine/threonine-protein phosphatase PP2A
Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.

Last Updated: 1999-03-03

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.