SPT4 / YGR063C Overview

Standard Name
SPT4 1 2
Systematic Name
Feature Type
ORF , Verified
Spt4p/5p (DSIF) transcription elongation factor complex subunit; Spt4/5 binds ssRNA in a sequence-specific manner and along with RNAP I and II regulates transcriptional elongation, RNA processing, quality control, and transcription-coupled repair; localizes to kinetochores and heterochromatin, influencing chromosomal dynamics and silencing; required for transcription through long trinucleotide repeats in ORFs and non-protein coding regions; accelerates senescence through rDNA instability 3 4 5 6 7 8 9 10 11 12 13
Name Description
SuPpressor of Ty's 2
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)
8567 +/- 2787


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.

View all SPT4 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Core RNA polymerase I and II-binding transcription factor that is a subunit of the DSIF complex; binds transcription elongation factor complexes and rDNA; involved in regulating DNA transcription, rRNA processing, transcription-coupled nucleotide-excision repair, and transcription elongation from RNA polymerase I and II promoters; also involved in 7-methylguanosine mRNA capping and mRNA splicing via the spliceosome, as well as chromatin organization and chromosome segregation; colocalizes with nuclear telomeric heterochromatin, mating-type region heterochromatin, rDNA heterochromatin, and the kinetochore

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated


Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.

Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Non-essential gene; null mutant exhibits three-budded morphology, fails to asymmetrically localize ASH1 mRNA and Ash1p to daughter cells, and is sensitive to heat, oxidative stress, and chemicals that affect nucleic acid metabolism; in large-scale studies, the null mutant has enlarged cells, shows reduced competitive fitness and chromosome instability, has a longer replicative lifespan but a shorter chronological lifespan, and is sensitive to heat, desiccation, acid pH, and anoxia; diploid homozygous null mutant requires myo-inositol and shows increased respiratory growth rate; null mutant displays altered sensitivity to a variety of chemicals
Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

460 total interactions for 262 unique genes

Physical Interactions

  • Affinity Capture-MS: 100
  • Affinity Capture-RNA: 12
  • Affinity Capture-Western: 14
  • Biochemical Activity: 2
  • Co-crystal Structure: 1
  • Co-localization: 1
  • Co-purification: 2
  • PCA: 1
  • Protein-peptide: 1
  • Proximity Label-MS: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 2

Genetic Interactions

  • Dosage Rescue: 1
  • Negative Genetic: 65
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 128
  • Positive Genetic: 11
  • Synthetic Growth Defect: 38
  • Synthetic Lethality: 49
  • Synthetic Rescue: 22
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.