VPS74 / YDR372C Overview

Standard Name
VPS74 1
Systematic Name
API1 9 , MNN3 10 11
Feature Type
ORF , Verified
Golgi PI4K effector, PtdIns4P sensor, and retention receptor; interacts with glycosyltransferases, and in the PtdIns4P-bound state mediates retention of these enzymes in the Golgi; PtdIns4P sensor that limits PI4K signaling and regulates lipid homeostasis, interacting with the catalytic domain of Sac1p, the major PtdIns4P phosphatase, directing dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; contributes to telomere function; ortholog of human GOLPH3 2 3 4 5 6 7 8
Name Description
Vacuolar Protein Sorting 1
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

VPS74/YDR372C is located on the right arm of chromosome IV between CTS2 chitinase and FRQ1 N-myristoylated calcium-binding protein; coding sequence is 1038 nucleotides long with 5 SNPs, two of which cause amino acid polymorphisms
Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Vps74p is 345 amino acids long, low in abundance, of average half-life; ubiquitinylated on 5 lysines, phosphorylated on 10 residues
Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)
4642 +/- 1918
Half-life (hr)


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.

View all VPS74 alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Mannosyltransferase- and phosphatidylinositol-4-phosphate binding protein involved in endoplasmic reticulum unfolded protein response, protein localization to Golgi, retrograde vesicle-mediated transport, and regulation of phosphatidylinositol dephosphorylation; localizes to nucleus, Golgi apparatus, and cytosol

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated
Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Non-essential gene; null exhibits increased replicative lifespan, poor anaerobic growth, small defect in vacuolar fragmentation, abnormal Golgi, decreased heat resistance, decreased competitive fitness, decreased utilization of urea as nitrogen source, decreased resistance to the toxicity of human amyloid, mislocalization of proteins to the vacuole, decreased metal resistance, decreased resistance to hygromycin B, tunicamycin, Calcofluor White, Congo Red, clotrimazole, various antibiotics and antifungals
Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

Vps74p interacts physically with proteins involved in Golgi vesicle transport; VPS74 interacts genetically with genes involved in Golgi vesicle transport and lipid metabolism

374 total interactions for 240 unique genes

Physical Interactions

  • Affinity Capture-MS: 15
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 4
  • Co-crystal Structure: 2
  • Co-fractionation: 1
  • Co-localization: 1
  • Co-purification: 1
  • Far Western: 1
  • PCA: 2
  • Proximity Label-MS: 2
  • Reconstituted Complex: 20
  • Two-hybrid: 5

Genetic Interactions

  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 14
  • Negative Genetic: 190
  • Phenotypic Enhancement: 37
  • Phenotypic Suppression: 8
  • Positive Genetic: 10
  • Synthetic Growth Defect: 45
  • Synthetic Lethality: 5
  • Synthetic Rescue: 5
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

VPS74 promoter is bound by Reb1p and Rpd3p in response to heat; VPS74 transcription is regulated by Tup1p
Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.

Last Updated: 2024-04-22

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.