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Complex: DNA-directed RNA polymerase III complex

Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.

Curated by
IntAct
ComplexAc
CPX-2660
Systematic Name
RET1:RPB5:RPB8:RPB10:RPC10:RPC11:RPC17:RPC19:RPC25:RPC31:RPC34:RPC37:RPC40:RPC53:RPC82:RPO26:RPO31
SGD ID
SGD:S000218119
Aliases
Pol III ; RNA Polymerase 3 ; RNA polymerase III
Description
Catalyzes the transcription of RNA from an DNA template by acting as a nucleotidyl transferase that polymerizes ribonucleotides at the 3-prime end of an RNA transcript. Responsible for the transcription of genes encoding small structured RNAs such as tRNAs, the 7 SL lncRNA, spliceosomal U6 snRNA and ribosomal 5S RNA. Pol III machinery recognizes conserved promoter elements located within the transcribed region, generally the box A and box B sequences, which contribute to the D- and T-loops in the tRNA structure. RPC34, RPC31 and RPC82 play a role in transcription initiation whereas the RPC37-RPC53 heterodimer is crucial for the correct recognition of the termination signals of class III genes. RPC11 is required for RNA cleavage. Pol III is capable of reinitiating transcription more rapidly on the same gene after the first transcription cycle without being released (facilitated reinitiation), resulting in a higher initiation efficiency; this appears to require an RPC11-dependent conformational change of Pol III. The core complex is believed to assemble in the cytoplasm before being transported to the nucleus.

Complex Diagram

This diagram displays the protein subunits (blue) of the complex and how they interact with each other. Interactions with other relevant participants such as small molecules (purple), sub-complexes (yellow), and other subunits (red) are also shown. If a participant appears in the diagram with no lines connected to it, then its specific interactions with other participants are currently unknown.

PDB Structure

When available from the Protein Data Bank (PDB), the physical structure of the macromolecular complex is displayed. Click on the image or link for further information about the physical structure.

2CKZ 2CKZ 5FJ8 5FJ8 5FJ9 5FJ9 5FJA 5FJA

Subunits

This table lists all participants of the complex (proteins, small molecules, nucleic acids, etc.) and their respective stoichiometry. A brief description is also provided for each participant in the table.

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Subunit Description Stoichiometry

Gene Ontology

Gene Ontology (GO) terms that describe the function of a complex, the biological process in which it participates, or its cellular location. Click on a term for its definition and other objects annotated to that term.

Shared Annotations

This diagram displays Gene Ontology terms (green) and subunits (blue) that are shared between the given macromolecular complex (black) and other yeast complexes (yellow). The shared GO terms or subunits can be visualized separately using the radio button at the bottom of the graph. Note: No diagram is shown ("No shared annotations") if there are less than 2 shared annotations (either GO terms or subunits of other complexes) between this complex and any other complexes.

References

List of references used specifically to curate the information on this page.

External Identifiers

EMDB: EMD-3178 | EMDB: EMD-3179 | EMDB: EMD-3180 | IntEnz: 2.7.7.6

Resources

IntAct | ModelArchive | PDB:2CKZ | PDB:5FJ8 | PDB:5FJ9 | PDB:5FJA