In isolated Saccharomyces cerevisiae cell walls (CWs) obtained without using denaturing agents, Bgl2p demonstrates hydrolysis of the laminaripentaose forming a disaccharide and generates the glucanosyltransglycosylase activity product, laminarioctaose, that confirms the results obtained previously for the enzyme isolated using denaturing conditions. Activity is detected in the CWs of the parental yeast strain and is not detected in the CWs of a strain with a deletion of BGL2 gene, that lacks Bgl2p. For the first time, Bgl2p activity was investigated under conditions as similar as possible to in vivo; the absence of the influence of high-molecular-weight polyphosphates, universal regulators of protein functioning, and resistance to the hydrolysis of trypsin together allow us to put forward a hypothesis about the significant protection of the Bgl2p molecule by the CW components.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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