Ded1p is an essential translation initiation factor that interacts with and is modulated by eIF4F. Ded1p also promotes the assembly and disassembly of stress granules, which contain non-translating mRNAs and translation initiation factors. As Ded1p affects both mRNA storage and translation initiation, regulation of Ded1p's function may affect whether mRNAs are translated or localized to cytoplasmic RNA granules. To identify regulators of Ded1p in Saccharomyces cerevisiae, we screened for overexpression suppressors of the severe growth defect conferred by high levels of Ded1p. We found that overexpression of HAT1, a lysine acetyltransferase, can suppress the growth defect conferred by overexpression of DED1, but we do not find evidence of direct acetylation. We demonstrate that HAT1 antagonizes the accumulation of P-bodies under short-term stresses. Under sustained glucose deprivation during stationary phase, strains lacking HAT1 form more stress granules and have a survival advantage. Given the genetic connection between HAT1 and DED1, we screened for other lysine acetyltransferases and deacetylases that have a genetic interaction with DED1, identifying several more of these post-translational modifiers as possible regulators of mRNA storage and/or translation. These results demonstrate connections between acetylation and the control of cytoplasmic mRNA localization.

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Journal Article

Authors

Panko AJ, Winters A, Rothbard NR, Hilliker AK

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