Squalene, as a natural triterpenoid exhibiting various physiological activities, is primarily extracted from shark liver oil. However, due to the declining shark populations and conservation concerns, the alternative methods for squalene production are needed. Synthetic biology offers the strategies for engineered yeasts capable of producing squalene. Although the extensive studies have been performed on squalene production by the engineered yeasts, a comprehensive systematic review summarizing these efforts is lack ing. Herein, firstly, this review describes the characteristics of the squalene biosynthesis pathway in yeast cells. Secondly, metabolic strategies for enhancing squalene production in yeasts are summarized. Thirdly, the advanced genetic engineering tools to boost squalene and other terpenoids production are investigated. Fourthly, the potential of emerging other yeasts for squalene synthesis is explored. Finally, the potential technologies applied in yeasts for improving squalene production are discussed. This review will provide comprehensive information on yeasts as chassis for squalene production, laying the foundation for squalene production in yeasts.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.
| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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